Epicardial spindle orientation controls cell entry into the myocardium.
Epicardial spindle orientation controls cell entry into the myocardium.
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DOI：
10.1016/j.devcel.2010.06.011
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发表时间：
2010-07-20
影响因子：
11.8
通讯作者：
Tallquist, Michelle D.
中科院分区：
文献类型：
--
作者：
Wu, Mingfu;Smith, Christopher L.;Hall, James A.;Lee, Ivy;Luby-Phelps, Kate;Tallquist, Michelle D.
在心脏形态发生过程中，心外膜细胞经历上皮间质转化（EMT）并迁移到心外膜下。人们对控制这一过程的细胞信号知之甚少。在这里，我们发现心外膜细胞表现出两种不同的有丝分裂纺锤体方向，平行或垂直于基底膜。经历垂直细胞分裂的细胞随后进入心肌。我们发现β-连环蛋白的丢失导致粘附连接的破坏和有丝分裂纺锤体方向的随机化。粘附连接的丧失也破坏了心外膜细胞内的 Numb 定位，并且心外膜中 Numb 和 Numblike 表达的破坏导致随机有丝分裂纺锤体方向。总而言之，这些数据表明定向有丝分裂纺锤体方向有助于心外膜 EMT，并暗示 β-连环蛋白和 Numb 的连接复合物参与纺锤体方向的调节。
During heart morphogenesis, epicardial cells undergo an epithelial to mesenchymal transition (EMT) and migrate into the subepicardium. The cellular signals controlling this process are poorly understood. Here, we show that epicardial cells exhibit two distinct mitotic spindle orientations, directed either parallel or perpendicular to the basement membrane. Cells undergoing perpendicular cell division subsequently enter the myocardium. We found that loss of β-catenin led to a disruption in adherens junctions and a randomization of mitotic spindle orientation. Loss of adherens junctions also disrupted Numb localization within epicardial cells and disruption of Numb and Numblike expression in the epicardium led to randomized mitotic spindle orientations. Taken together, these data suggest that directed mitotic spindle orientation contributes to epicardial EMT and implicate a junctional complex of β-catenin and Numb in the regulation of spindle orientation.
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