The Airway Antigen Sampling System: Respiratory M Cells as an Alternative Gateway for Inhaled Antigens
The Airway Antigen Sampling System: Respiratory M Cells as an Alternative Gateway for Inhaled Antigens
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DOI：
10.4049/jimmunol.0903794
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发表时间：
2011-04-01
影响因子：
4.4
通讯作者：
Kiyono, Hiroshi
中科院分区：
文献类型：
--
作者：
Kim, Dong-Young;Sato, Ayuko;Kiyono, Hiroshi
在这项研究中，我们证明了一个新的气道Ag采样点，通过分析组织切片的小鼠鼻腔通道。我们揭示了呼吸道M细胞的存在，它有能力采取了卵清蛋白和重组鼠伤寒沙门氏菌表达GFP，在覆盖单层上皮的鼻甲。这些M细胞也能够采取呼吸道病原体A组链球菌鼻攻击后。DNA结合/分化抑制剂2（Id 2）缺陷型小鼠（淋巴组织缺陷，包括鼻咽相关淋巴组织）的鼻上皮中M细胞簇的频率与同窝Id 2（+/-）小鼠相似。重组沙门氏菌ToxC或A组链球菌经鼻免疫后，Id 2（-/-）小鼠和Id 2（+/-）小鼠中Ag特异性抗体的滴度一样高，表明呼吸道M细胞能够采样吸入的细菌Ag以启动Ag特异性免疫应答。综上所述，这些发现表明，呼吸道M细胞作为鼻咽相关淋巴组织独立的替代网关Ag采样和随后的诱导Ag特异性免疫反应在上呼吸道。免疫学杂志，2011，186：4253-4262。
In this study, we demonstrated a new airway Ag sampling site by analyzing tissue sections of the murine nasal passages. We revealed the presence of respiratory M cells, which had the ability to take up OVA and recombinant Salmonella typhimurium expressing GFP, in the turbinates covered with single-layer epithelium. These M cells were also capable of taking up respiratory pathogen group A Streptococcus after nasal challenge. Inhibitor of DNA binding/differentiation 2 (Id2)-deficient mice, which are deficient in lymphoid tissues, including nasopharynx-associated lymphoid tissue, had a similar frequency of M cell clusters in their nasal epithelia to that of their littermates, Id2(+/-) mice. The titers of Ag-specific Abs were as high in Id2(-/-) mice as in Id2(+/-) mice after nasal immunization with recombinant Salmonella-ToxC or group A Streptococcus, indicating that respiratory M cells were capable of sampling inhaled bacterial Ag to initiate an Ag-specific immune response. Taken together, these findings suggest that respiratory M cells act as a nasopharynx-associated lymphoid tissue-independent alternative gateway for Ag sampling and subsequent induction of Ag-specific immune responses in the upper respiratory tract. The Journal of Immunology, 2011, 186: 4253-4262.