MITOCHONDRIAL-DNA OF THE HUMAN MALARIAL PARASITE PLASMODIUM-FALCIPARUM
MITOCHONDRIAL-DNA OF THE HUMAN MALARIAL PARASITE PLASMODIUM-FALCIPARUM
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DOI：
10.1016/0166-6851(88)90140-5
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发表时间：
1988-10-01
影响因子：
1.5
通讯作者：
WILLIAMSON, DH
中科院分区：
文献类型：
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作者：
GARDNER, MJ;BATES, PA;WILLIAMSON, DH
用含溴化乙锭或2“，6-二脒基-2-苯基吲哚的CsCl梯度等密度离心法从恶性疟原虫总DNA中分离出共价闭合环状DNA分子。圆形分子的平均轮廓长度为11.1 ± 1.5。0.5μ m，类似于先前从猴疟原虫诺氏疟原虫分离的类似分子。这两种环状分子具有相当大的序列同源性和保守的限制性位点。恶性疟原虫分子的一个936 bp片段的核苷酸序列通过数据库同源性搜索被确定和鉴定为线粒体小rRNA亚基的一部分，从而证实了两种疟疾物种的环状DNA的线粒体起源。
Covalently closed circular DNA molecules were isolated from Plasmodium falciparum total DNA by isopycnic centrifugation in CsCl gradients containing either ethidium bromide or 2'',6-diamidino-2-phenylindole. The circular molecules had an average contour length of 11.1 .+-. 0.5 .mu.m, similar to the analogous molecules previously isolated from the simian malaria parasite P. knowlesi. Both circular molecules shared considerable sequence homology and conserved restriction sites. The nucleotide sequence of one 936 bp fragment of the P. falciparum molecule was determined and identified, by a data base homology search, as part of a mitochondrial small rRNA subunit, thus confirming the mitochondrial origin of the circular DNAs of both malarial species.