FT5a interferes with the Dt1-AP1 feedback loop to control flowering time and shoot determinacy in soybean
FT5a interferes with the Dt1-AP1 feedback loop to control flowering time and shoot determinacy in soybean
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FT5a 干扰 Dt1-AP1 反馈环路以控制大豆开花时间和芽决定性
DOI：
10.1111/jipb.13070
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发表时间：
2021
影响因子：
11.4
通讯作者：
Kong Fanjiang
中科院分区：
文献类型：
--
作者：
Yue Lin;Li Xiaoming;Fang Chao;Chen Liyu;Yang Hui;Yang Jie;Chen Zhonghui;Nan Haiyang;Chen Linnan;Zhang Yuhang;Li Haiyang;Hou Xingliang;Dong Zhicheng;Weller James L;Abe Jun;Liu Baohui;Kong Fanjiang
开花时间和茎的生长习性决定了大豆的花序结构，而花序结构又反过来影响种子产量。Dt 1是拟南芥终末花1（TFL 1）的同源物，是茎生长习惯的主要控制者，但其潜在的分子机制仍不清楚。在这里，我们表明，Dt 1影响节点数和株高，以及开花时间，在大豆长日照条件下。bZIP转录因子FDc 1与Dt 1物理相互作用，FDc 1-Dt 1复合物直接抑制APETALA 1（AP 1）的表达。我们认为FT 5a通过与FDc 1的竞争性相互作用抑制Dt 1活性，并直接上调AP 1。此外，AP 1抑制Dt 1的表达，直接结合到Dt 1启动子，表明AP 1和Dt 1形成一个抑制性的调节反馈回路，以确定芽顶端分生组织的命运。这些发现为深入研究Dt 1和FT 5a在大豆茎生长习性和开花时间调控中的作用提供了新的视角，而茎生长习性和开花时间决定了大豆的适应性和产量。
Flowering time and stem growth habit determine inflorescence architecture in soybean, which in turn influences seed yield. Dt1, a homolog of Arabidopsis TERMINAL FLOWER 1 (TFL1), is a major controller of stem growth habit, but its underlying molecular mechanisms remain unclear. Here, we demonstrate that Dt1 affects node number and plant height, as well as flowering time, in soybean under long-day conditions. The bZIP transcription factor FDc1 physically interacts with Dt1, and the FDc1-Dt1 complex directly represses the expression of APETALA1 (AP1). We propose that FT5a inhibits Dt1 activity via a competitive interaction with FDc1 and directly upregulates AP1. Moreover, AP1 represses Dt1 expression by directly binding to the Dt1 promoter, suggesting that AP1 and Dt1 form a suppressive regulatory feedback loop to determine the fate of the shoot apical meristem. These findings provide novel insights into the roles of Dt1 and FT5a in controlling the stem growth habit and flowering time in soybean, which determine the adaptability and grain yield of this important crop.