Electrically conductive nanofibers with highly oriented structures and their potential application in skeletal muscle tissue engineering
Electrically conductive nanofibers with highly oriented structures and their potential application in skeletal muscle tissue engineering
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DOI：
10.1016/j.actbio.2012.10.024
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发表时间：
2013-03-01
影响因子：
9.7
通讯作者：
Chen, Yuan-Hsiang
中科院分区：
文献类型：
--
作者：
Chen, Mei-Chin;Sun, Yu-Chin;Chen, Yuan-Hsiang
支架设计的最新趋势集中在能够为特定细胞类型提供适当指导线索的材料上，以调节细胞行为。在这项研究中，我们开发了高度定向和导电的纳米纤维，可以同时为电池提供地形和电学信号。随后，对其作为骨骼肌组织工程功能支架的潜力进行了研究。在聚集区引入外加磁场，制备了由聚苯胺(PANI)和聚己内酯(PCL)组成的有序纳米纤维。在PCL纤维中掺入PANI后，电导率从纯PCL纤维的不可检测水平显著提高到含3wt%PANI(PCL/PANI-3)纤维的63.6+/-6.6ms cm(-1)。为研究电纺丝支架对骨骼肌成肌细胞分化的协同作用，将小鼠C2C12成肌细胞分别接种于随机PCL(R-PCL)、定向PCL(A-PCL)、随机PCL/PANI-3(R-PCL/PANI)和定向PCL/PANI-3(A-PCL/PANI)纳米纤维上。我们的结果表明，与R-PCL支架相比，排列的纳米纤维(A-PCL和A-PCL/PANI)可以引导成肌细胞定向并促进肌管的形成(即肌管数量分别增加约40%和80%)。此外，与非导电的A-PCL支架或R-PCL/PANI相比，导电的A-PCL/PANI纳米纤维进一步促进了肌管的成熟(即融合指数和成熟指数分别增加了约30%和23%或15%和18%)。这些结果表明，两种引导线索的联合作用比单独的引导线索更有效，这表明A-PCL/PANI纳米纤维可能用于骨骼肌再生。(C)2012 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Recent trends in scaffold design have focused on materials that can provide appropriate guidance cues for particular cell types to modulate cell behavior. In this study highly aligned and electrically conductive nanofibers that can simultaneously provide topographical and electrical cues for cells were developed. Thereafter their potential to serve as functional scaffolds for skeletal muscle tissue engineering was investigated. Well-ordered nanofibers, composed of polyaniline (PANi) and poly(epsilon-caprolactone) (PCL), were electrospun by introducing an external magnetic field in the collector region. Incorporation of PANi into PCL fibers significantly increased the electrical conductivity from a non-detectable level for the pure PCL fibers to 63.6 +/- 6.6 mS cm(-1) for the fibers containing 3 wt.% PANi (PCL/PANi-3). To investigate the synergistic effects of topographical and electrical cues using the electrospun scaffolds on skeletal myoblast differentiation, mouse C2C12 myoblasts were cultured on random PCL (R-PCL), aligned PCL (A-PCL), random PCL/PANi-3 (R-PCL/PANi) and aligned PCL/PANi-3 (A-PCL/PANi) nanofibers. Our results showed that the aligned nanofibers (A-PCL and A-PCL/PANi) could guide myoblast orientation and promote myotube formation (i.e. approximately 40% and 80% increases in myotube numbers) compared with R-PCL scaffolds. In addition, electrically conductive A-PCL/PANi nanofibers further enhanced myotube maturation (i.e. approximately 30% and 23% or 15%and 18% increases in the fusion and maturation indices) compared with non-conductive A-PCL scaffolds or R-PCL/PANi. These results demonstrated that a combined effect of both guidance cues was more effective than an individual cue, suggesting a potential use of A-PCL/PANi nanofibers for skeletal muscle regeneration. (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.