Elements and organic substances in epiretinal proliferative tissue excised during vitreous surgery: analysis by time-of-flight secondary-ion mass spectrometry.
Elements and organic substances in epiretinal proliferative tissue excised during vitreous surgery: analysis by time-of-flight secondary-ion mass spectrometry.
复制标题
玻璃体手术切除的视网膜前增殖组织中的元素和有机物质：飞行时间二次离子质谱分析。
DOI：
10.1093/jmicro/52.3.349
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Y. Ohashi
中科院分区：
文献类型：
--
作者：
Y. Kishikawa;H. Gong;T. Kitaoka;T. Amemiya;K. Takaya;M. Tozu;T. Hoshi;Y. Ohashi
我们用飞行时间二次离子质谱（TOF-SIMS）检测了增生性糖尿病视网膜病变或增生性玻璃体视网膜病变患者玻璃体增生组织中的各种物质。用TOF-SIMS测定元素、维生素和脂肪酸的量，然后计算每种元素和有机物的溅射离子数与溅射离子总数的平均比率。增生性糖尿病视网膜病变组的Fe、Ca、Al、Zn和Cu离子计数占飞溅离子总数的平均比值明显大于增生性玻璃体视网膜病变组。增殖性糖尿病视网膜病变中维生素A片段-1和棕榈酸离子计数与飞溅离子总数的平均比率显著大于增殖性玻璃体视网膜病变。糖尿病和非糖尿病视网膜前增生组织含有有机物质的事实表明，这些增生组织来源于视网膜，特别是深层视网膜，可能是感光细胞和/或视网膜色素上皮。Zn和Cu的存在也表明了相同的过程。富含有机物质的糖尿病增殖组织被认为比非糖尿病组织生长得更多，因为维生素A和E以及脂肪酸是维持细胞结构和组织增殖所必需的。通过TOF-SIMS分析生物样品有望提供有价值的信息。
We examined with time-of-flight secondary-ion mass spectrometry (TOF-SIMS) various substances in vitreous proliferative tissues excised from patients with proliferative diabetic retinopathy or proliferative vitreoretinopathy. Amounts of elements, vitamins and fatty acids were measured with TOF-SIMS and then the average ratio of the count of spattered ions of each element and organic substance to the total count of spattered ions was calculated. The average ratios of Fe, Ca, Al, Zn and Cu ion counts to the total count of spattered ions were significantly larger in proliferative diabetic retinopathy than in proliferative vitreoretinopathy. The average ratios of each of vitamin A fragment-1 and palmitic acid ion counts to the total count of spattered ions were significantly larger in proliferative diabetic retinopathy than in proliferative vitreoretinopathy. The fact that diabetic and non-diabetic preretinal proliferative tissues contain organic substances suggests that these proliferative tissues are derived from the retina, especially the deep retina, probably the photoreceptor cell and/or retinal pigment epithelium. The presence of Zn and Cu also suggest the same process. Diabetic proliferative tissues rich in organic substances are thought to grow more than do non-diabetic tissues, because vitamins A and E, and fatty acids are necessary for the maintenance of cell structure and the proliferation of tissue. Analysis of biological specimens by TOF-SIMS is expected to provide valuable information.