Detection of immune complexes is not independent of detection of antibodies in Lyme disease patients and does not confirm active infection with Borrelia burgdorferi.
Detection of immune complexes is not independent of detection of antibodies in Lyme disease patients and does not confirm active infection with Borrelia burgdorferi.
复制标题
免疫复合物的检测并不独立于莱姆病患者抗体的检测，并且不能确认伯氏疏螺旋体的活动性感染。
DOI：
10.1128/cdli.12.9.1036-1040.2005
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Philipp,MarioT
中科院分区：
文献类型：
--
作者：
Marques,AdrianaR;Hornung,RonaldL;Dally,Len;Philipp,MarioT
TheBorrelia burgdorferi-specific immune complex (IC) test, which uses polyethylene glycol (PEG) precipitation to isolate ICs from serum, has been used as a research test in the laboratory diagnosis of early Lyme disease (LD) and has been proposed as a marker of active infection. We examined whetherB. burgdorferi-specific antibodies were present within PEG-precipitated ICs (PEG-ICs) in patients with LD, posttreatment Lyme disease syndrome, and controls, including individuals who received the outer surface protein A (OspA) vaccine. Using aB. burgdorferiwhole-cell enzyme-linked immunosorbent assay (ELISA), we obtained positive PEG-IC results not only in patients with a history of LD, but also in individuals vaccinated with OspA vaccine. The frequency of positive PEG-IC ELISAs in OspA vaccinees was significantly higher with ELISA-reactive than with ELISA-negative unprocessed serum samples (P= 0.001), demonstrating dependency between the tests. Similar results were found using samples from rhesus macaques infected withB. burgdorferi, uninfected macaques vaccinated with OspA, and controls. Therefore, testing for the presence of antibodies againstB. burgdorferiin PEG-IC preparations is not more likely to reflect active infection than testing in unprocessed serum and should not be used in individuals who received the OspA vaccine.
