Application of novel PCR strategies to amplify and sequence glucose transporters in canine brain.
Application of novel PCR strategies to amplify and sequence glucose transporters in canine brain.
复制标题
应用新型 PCR 策略对犬脑中葡萄糖转运蛋白进行扩增和测序。
DOI：
10.1007/978-1-4615-2920-0_4
复制
发表时间：
1993
影响因子：
--
通讯作者：
Drewes,LR
中科院分区：
文献类型：
--
作者：
Borson,ND;Salo,WL;Drewes,LR
葡萄糖在哺乳动物大脑中的转运是非常重要的，不仅对生长和发育，而且为功能活动提供能量。在哺乳动物组织中，葡萄糖部分通过葡萄糖转运蛋白家族的成员跨质膜转运。该家族的五种高度保守的同种型（Glutl-Glut 5）已在各种物种和组织中被鉴定，并且所有五个成员被认为是促进葡萄糖扩散的整合膜蛋白。1，2 Glutl和Glut 3同种型已在几个物种的脑中被检测到，3- 8但出现了关于其他葡萄糖转运蛋白家族成员是否也表达的问题。此外，还存在一种可能性，即一个尚未确定的家族成员可能在大脑中表达。因此，需要一种快速可靠的方法来确定哪些葡萄糖转运蛋白同种型在各种物种和组织中表达。
The transport of glucose in the brain of mammalian species is extremely important, not only for growth and development, but to provide energy for functional activity. Glucose is transported across plasma membranes in mammalian tissues in part by members of a family of glucose transporters. Five highly conserved isoforms of this family (Glutl-Glut5) have been identified in various species and tissues, and all five members are believed to be integral membrane proteins that facilitate glucose diffusion.1,2 The Glutl and Glut3 isoforms have been detected in brain in several species,3-8but a question arises as to whether or not other glucose transporter family members are also expressed. Also, the possibility exists that an as yet unidentified family member may be expressed in brain. Therefore, a rapid and reliable method of determining which glucose transporter isoforms are expressed in various species and tissues is desirable.