The use of phospholipid fatty acid analysis to estimate the changes in microbial community structure during wood decay in forests
The use of phospholipid fatty acid analysis to estimate the changes in microbial community structure during wood decay in forests
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DOI：
10.2488/jwrs.54.289
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
M. Matsushita;Ayami Hato;S. Meguro
中科院分区：
文献类型：
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作者：
M. Matsushita;Ayami Hato;S. Meguro
采用磷脂脂肪酸(PLFA)分析方法，对埋藏在杉木人工林和阔叶林土壤中的Sugi和Konara木材样品在腐解过程中微生物群落结构的变化进行了研究。聚类分析的结果表明，PLFA法估算的微生物群落结构按样地的树种分为两类，而不是按森林植被进行分类。在Konara木材样品中，以真菌来源的PLFA衡量的真菌生物量指数在较高水平上保持不变，革兰氏阳性细菌生物量随着腐烂的进行而增加。各微生物生物量与木材腐烂程度呈正相关。在杉木木材样品中，真菌、革兰氏阳性细菌和放线菌群的PLFAs在木材腐烂过程中有所增加，但真菌生物量与木材腐烂程度之间没有相关性。这些结果表明，细菌和放线菌群而不是真菌群在杉木样品的腐烂过程中起着重要作用。
Phospholipid fatty acid (PLFA) analysis was used to estimate the changes in microbial community structure during decompositions of sugi and konara wood samples buried in soil of sugi plantation or broad-leaved forest. As the result of cluster analysis, the microbial community structure estimated by PLFA composition was classified in two groups by the wood species of sample, not by the forest vegetation. In konara wood samples, the index of fungal biomass measured as PLFA of fungal origin was held constant at a higher level, and the amount of gram positive bacterial biomass increased as the decay proceeded. The amount of each microbial biomass was positively correlated with the degree of wood decay. In sugi wood samples, PLFAs chosen as indexes of fungal, gram positive bacterial and actinomyces groups increased during wood decay, but no correlation was found between the amount of fungal biomass and the degree of wood decay. These results suggested that bacterial and actinomyces groups other than the fungal group would play a significant role in the decay of sugi wood samples.