Live Imaging of Planarian Membrane Potential Using DiBAC4(3).
Live Imaging of Planarian Membrane Potential Using DiBAC4(3).
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DOI：
10.1101/pdb.prot5055
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发表时间：
2008-10-01
期刊：
影响因子：
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通讯作者：
Levin, Michael
中科院分区：
文献类型：
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作者：
Oviedo, Nestor J;Nicolas, Cindy L;Levin, Michael
引言本方案描述了如何使用阴离子膜电压报告染料DiBAC（4）（3）来产生活的真涡虫细胞膜电位的图像。这些图像定性地揭示了生物体不同区域的时间平均膜电位的变化。由于实验性治疗，这些图像的变化揭示了特定治疗如何影响该生理参数。这种方法是对标准电生理技术的巨大改进，由于细胞尺寸小和细胞数量大，标准电生理技术不能用于了解整个蠕虫或再生片段的电特性。当执行适当的控制时，这种技术是收集生理数据的一种非常强大且简单的方法。
INTRODUCTIONThis protocol describes how to use the anionic membrane voltage-reporting dye DiBAC(4)(3) to generate images of cell membrane potential in live planarians. These images qualitatively reveal variations in time-averaged membrane potential across different regions of the organism. Changes in these images due to experimental treatments reveal how the particular treatment affects this physiological parameter. This method is a great improvement over standard electrophysiological techniques, which cannot be used to gain an understanding of the electrical properties of an entire worm or a regenerating fragment, due to small cell size and large cell number. When the proper controls are performed, this technique is a very powerful and simple way to gather physiologic data.