Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches
Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches
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DOI：
10.1007/978-1-60761-691-7_18
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Ding, Sheng
中科院分区：
文献类型：
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作者：
Li, Wenlin;Ding, Sheng
尽管自1981年以来，已经从小鼠中建立了胚胎干细胞（ESC），但从包括大鼠在内的各种其他哺乳动物中获得其对应物的尝试尚未成功。最近，诱导多能干细胞（iPSC）已产生从小鼠和人类体细胞通过遗传转导。我们已经成功地建立了新的大鼠iPSCs（riPSC），其可以通过LIF和ALK 5抑制剂、GSK3抑制剂和MEK抑制剂的混合物均匀地维持。riPSC具有常规的小鼠ESC特征，最重要的是可以广泛地促进嵌合体。我们还产生了具有“小鼠胚胎干细胞样”特征的新型人iPSC（hiPSC），其可以在MEK抑制剂和ALK 5抑制剂存在的情况下令人惊讶地维持在培养物中。
Although embryonic stem cells (ESCs) have been established from mice since 1981, attempts to derive its counterparts from various other mammals, including rats, have not succeeded. Recently, induced pluripotent stem cells (iPSCs) have been generated from both mouse and human somatic cells by genetic transduction. We had successfully established novel rat iPSCs (riPSCs), which can be homogenously maintained by LIF and a cocktail of ALK5 inhibitor, GSK3 inhibitor and MEK inhibitor. riPSCs share conventional mouse ESC characteristics and most importantly can contribute extensively to chimeras. We also generated novel human iPSCs (hiPSCs) with "mouse ESC-like" characteristics, which can be surprisingly maintained in culture in the presence of MEK inhibitor and ALK5 inhibitor.