GLUCONEOGENESIS IN ISOLATED CHICKEN (GALLUS-DOMESTICUS) LIVER-CELLS
GLUCONEOGENESIS IN ISOLATED CHICKEN (GALLUS-DOMESTICUS) LIVER-CELLS
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DOI：
10.1016/0305-0491(79)90029-4
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发表时间：
1979-01-01
影响因子：
2.2
通讯作者：
LEVEILLE, GA
中科院分区：
文献类型：
--
作者：
BRADY, LJ;ROMSOS, DR;LEVEILLE, GA
研究了离体禽肝细胞糖异生现象。产糖率最高的是乳酸，其次是二羟丙酮、甘油醛和果糖。丙氨酸转化为葡萄糖的速率只有乳酸的4%左右。向肝细胞中添加10 mM山梨醇、木糖醇或乙醇可使丙酮酸产生的葡萄糖增加25-40%，而添加甘油仅增加9%。添加。beta。羟基丁酸对乳酸和丙酮酸的葡萄糖生成没有影响。辛酸盐的添加对丙酮酸盐生成葡萄糖没有影响，但在5 mM处抑制了乳酸盐生成葡萄糖。不同底物生成葡萄糖的差异表明鸡与大鼠和豚鼠在葡萄糖生成模式上存在一些基本差异。
Gluconeogenesis was studied in isolated avian hepatocytes. The highest rate of glucose production obtained was from lactate, followed by dihydroxyacetone, glyceraldehyde, and fructose. Alanine was converted to glucose at only about 4% the rate of lactate. Addition of 10 mM sorbitol, xylitol, or ethanol to the hepatocytes increased glucose production from pyruvate 25-40%, while glycerol addition increased it only 9%. Addition of .beta.-hydroxybutyrate had no effect on glucose production from lactate or pyruvate. Addition of octanoate had no effect on glucose production from pyruvate, but depressed it from lactate at 5 mM. Differences in the formation of glucose from various substrates suggest some basic differences in the mode of glucose production between the chick and the rat and guinea-pig.