Tissue-specific and inducible Cre-mediated recombination in the gut epithelium
Tissue-specific and inducible Cre-mediated recombination in the gut epithelium
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DOI：
10.1002/gene.20042
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发表时间：
2004-07-01
期刊：
影响因子：
1.5
通讯作者：
Robine, S
中科院分区：
文献类型：
--
作者：
El Marjou, F;Janssen, KP;Robine, S
我们产生了两个互补的系统Cre介导的重组靶基因在小鼠消化上皮细胞和测试他们与Cre报告小鼠品系。Cre在鼠绒毛蛋白基因（vil-Cre）的9 kb调控区的控制下表达。在胚胎第9天内脏内胚层中开始了遗传重组，在E12.5在整个肠上皮中开始了遗传重组，但在其他组织中没有。Cre表达在整个成年期都保持不变。此外，创建了携带在绒毛蛋白启动子控制下表达的他莫昔芬依赖性Cre重组酶（vil-Cre-ERT 2）的转基因小鼠，以进行靶向时空控制的体细胞重组。经过他莫昔芬治疗后，整个消化上皮都可以检测到重组。重组位点持续60天后，他莫昔芬管理，尽管快速肠细胞更新，表明上皮祖细胞已被靶向。villin-Cre和villin-Cre-ERT 2小鼠为研究发育和成年肠道中的细胞谱系分配和基因功能提供了有价值的工具。(C)2004 Wiley-Liss，Inc.
We generated two complementary systems for Cre-mediated recombination of target genes in the mouse digestive epithelium and tested them with a Cre-reporter mouse strain. Cre was expressed under the control of a 9 kb regulatory region of the murine villin gene (vil-Cre). Genetic recombination was initiated at embryonic day (E) 9 in the visceral endoderm, and by E12.5 in the entire intestinal epithelium, but not in other tissues. Cre expression was maintained throughout adulthood. Furthermore, transgenic mice bearing a tamoxifen-dependent Cre recombinase (vil-Cre-ERT2) expressed under the control of the villin promoter were created to perform targeted spatiotemporally controlled somatic recombination. After tamoxifen treatment, recombination was detectable throughout the digestive epithelium. The recombined locus persisted for 60 days after tamoxifen administration, despite rapid intestinal cell renewal, indicating that epithelial progenitor cells had been targeted. The villin-Cre and villin-Cre-ERT2 mice provide valuable tools for studies of cell lineage allocation and gene function in the developing and adult intestine. (C) 2004 Wiley-Liss, Inc.