Imaging of Molecular Interaction Between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes
Imaging of Molecular Interaction Between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes
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CCN 蛋白及其结合伴侣之间的分子相互作用成像：软骨细胞中 CCN2 和 Rab14 之间相互作用的原位邻近连接分析
DOI：
10.1007/978-1-0716-2744-0_4
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发表时间：
2022
影响因子：
--
通讯作者：
Takigawa Masaharu
中科院分区：
文献类型：
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作者：
Hoshijima Mitsuhiro;Aoyama Eriko;Kamioka Hiroshi;Takigawa Masaharu
原位邻位连接测定（PLA）使得能够可视化固定细胞中的蛋白质相互作用。它是研究内源性表达蛋白质的蛋白质-蛋白质结合的强有力的方法。为了证实软骨细胞中CCN 2和Rab 14 GT3（Rab 14）之间的结合，我们使用软骨细胞HCS-2/8细胞进行PLA。本章中的方案介绍了一种优化的技术，用于使用PLA在固定的细胞中可视化CCN 2和Rab 14的细胞内相互作用。
An in situ proximity ligation assay (PLA) enables visualization of protein interactions in fixed cells. It is a powerful method for investigating protein-protein binding of endogenously expressed proteins. To confirm binding between CCN2 and Rab14 GTPase (Rab14) in chondrocytes, we performed a PLA using chondrocytic HCS-2/8 cells. The protocol in this chapter introduces an optimized technique for visualizing intracellular interactions of CCN2 and Rab14 in fixed cells using a PLA.