Expression of kallikrein-related peptidase 4 in dental and non-dental tissues.
Expression of kallikrein-related peptidase 4 in dental and non-dental tissues.
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DOI：
10.1111/j.1600-0722.2011.00834.x
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发表时间：
2011-12
影响因子：
1.9
通讯作者：
Hu JC
中科院分区：
文献类型：
--
作者：
Simmer JP;Richardson AS;Smith CE;Hu Y;Hu JC
激肽释放酶4（Klk 4）对于适当的牙釉质形成至关重要。Klk 4基因敲除小鼠和两个KLK 4等位基因缺陷的人有明显的釉质缺陷，没有其他明显的表型。据报道，Klk 4 mRNA或蛋白存在于牙齿以外的组织中，包括前列腺、卵巢、肾、肝和唾液腺。在这项研究中，我们使用Klk 4敲除/NLS-lacZ敲入小鼠，使用β-半乳糖苷酶组织化学来测定Klk 4表达。孵育5小时用于检测具有最小内源性背景的Klk 4表达，而易受假阳性影响的过夜孵育用于寻找痕量Klk 4表达。通过X-gal组织化学检查出生后第5、6、7、8和14天发育中的上颌磨牙，出生后第14天发育中的下颌切牙，以及成年野生型和Klk 4lacZ/lacZ小鼠的选定非牙组织。孵育5 h后，在Klk 4lacZ/lacZ小鼠磨牙和切牙的成熟期成釉细胞核中观察到特异性Xgal染色，并在唾液腺导管和前列腺上皮斑块的细胞核中检测到弱染色。我们得出结论，Klk 4主要是牙齿特异性蛋白酶，在下颌下唾液腺和前列腺中低表达，并且在肝脏、肾脏、睾丸、卵巢、输卵管、附睾和输精管中没有检测到表达。
Kallikrein 4 (Klk4) is critical for proper dental enamel formation. Klk4 null mice and persons with two defective KLK4 alleles have obvious enamel defects, with no other apparent phenotype. Klk4 mRNA or protein is reported to be in tissues besides teeth, including prostate, ovary, kidney, liver, and salivary gland. In this study we used the Klk4 knockout/NLS-lacZ knockin mouse to assay Klk4 expression using ß-galactosidase histochemistry. Incubations for 5 h were used to detect Klk4 expression with minimal endogenous background, while overnight incubations susceptible to false positives were used to look for trace Klk4 expression. Developing maxillary molars at postnatal days 5, 6, 7, 8, and 14, developing mandibular incisors at postnatal day 14, and selected non-dental tissues from adult wild-type and Klk4lacZ/lacZ mice were examined by X-gal histochemistry. After 5 h incubation, Xgal staining was observed specifically in the nuclei of maturation stage ameloblasts in molars and incisors from Klk4lacZ/lacZ mice and was detected weakly in nuclei of salivary gland ducts and patches of prostate epithelia. We conclude that Klk4 is predominantly a tooth-specific protease with low expression in submandibular salivary gland and prostate, and with no detectable expression in liver, kidney, testis, ovary, oviduct, epididymis, and vas deferens.
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影响因子：
9.2
作者：
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10.1098/rspb.2010.1280
发表时间：
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通讯作者：
Springer, Mark S.