Establishment and characterization of a primary androgen-responsive African-American prostate cancer cell line, E006AA
Establishment and characterization of a primary androgen-responsive African-American prostate cancer cell line, E006AA
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DOI：
10.1002/pros.20053
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发表时间：
2004-07-01
期刊：
影响因子：
2.8
通讯作者：
Rayford, W
中科院分区：
文献类型：
--
作者：
Koochekpour, S;Maresh, GA;Rayford, W
背景人前列腺癌细胞系的建立对于促进我们对与疾病的起始和进展相关的复杂过程的理解至关重要。在本研究中，我们建立了一个原代非洲裔美国人前列腺癌细胞系（E006 AA）及其相关基质细胞（S 006 AA）。E006 AA细胞系是从临床局限性前列腺癌患者中建立的自发永生化细胞。使用雄激素依赖性生长和敏感性测定、Western分析、RT-PCR/实时PCR、细胞遗传学分析和裸鼠致瘤性完成细胞的广泛表征。E006 AA细胞系表现出雄激素依赖性生长，表达PSA和雄激素受体（AR），AR的外显子1具有26个CAG重复。细胞遗传学分析显示超三倍体核型，在染色体5，6，8，10，17，20，21和未知来源的标记染色体以及染色体4，5，8，9，11-14，18和20中的结构异常。该细胞系在裸鼠中不具有致瘤性。从同一肿瘤标本中分离的S 006 AA细胞株也表达AR，并显示前列腺间质平滑肌细胞的形态学特征。这些细胞系是第一个可用的原代上皮细胞和基质细胞来自非洲裔美国人的器官局限性前列腺癌患者，并与其他已建立的细胞系，可以提供一个体外模型系统，以研究前列腺癌的早期转化事件。前列腺60：141-152，2004年。(C)2004威利-利斯公司
BACKGROUND. Establishment of human prostate cancer cell lines is essential to advance our understanding of complex processes associated with the initiation and progression of the disease. In the present study, we report the establishment of a primary African-American prostate cancer cell line (E006AA) as well as its associated stromal cells (S006AA).METHODS. E006AA cell line was established as a spontaneously immortalized cells from a patient with a clinically localized prostate cancer. Extensive characterization of the cells was accomplished using androgen-dependent growth and sensitivity assays, Western analyses, RT-PCR/real-time PCR, cytogenetic analyses, and tumorigenicity in nude mice.RESULTS. E006AA cell line shows androgen-dependent growth, expresses PSA and the androgen receptor (AR) with 26 CAG repeats in exon 1 of AR. Cytogenetic analyses revealed a hypertriploid karyotype with additional numerical gains in chromosomes 5, 6, 8, 10, 17, 20, 21 and a marker chromosome of unknown origin as well as structural abnormalities in chromosomes 4,5,8,9, 11-14, 18, and 20. This cell line is not tumorigenic in nude mice. S006AA cell line, isolated from the same tumor specimen, also expresses AR and shows the morphological characteristics of smooth muscle cells of prostatic stroma.CONCLUSIONS. These cell lines are the first available primary epithelial and stromal cells derived from an African-American patient with organ-confined prostate cancer and in conjunction with other established cell lines, could provide an in vitro model system to investigate early transforming events in prostate cancer. Prostate 60: 141-152, 2004. (C) 2004 Wiley-Liss, Inc.