Differentiation of human mesenchymal stem cells into mesangial cells in post-glomerular injury murine model
Differentiation of human mesenchymal stem cells into mesangial cells in post-glomerular injury murine model
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DOI：
10.1080/00313020701716367
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发表时间：
2008-01-01
期刊：
影响因子：
4.5
通讯作者：
Leong, Chooi-Fun
中科院分区：
文献类型：
--
作者：
Wong, Chee-Yin;Cheong, Soon-Keng;Leong, Chooi-Fun
目的：成人骨髓含有一群间充质干细胞（MSC），有助于骨、软骨、肌肉、肌腱和脂肪等组织的再生。近年来研究发现，成人骨髓中存在功能性干细胞，它们可参与肾脏重塑或损伤肾小球的重建，尤其是肾小球系膜细胞。本研究的目的是检测从人骨髓中分离的MSC在肾小球损伤的无胸腺小鼠中向肾小球系膜细胞分化的能力。人骨髓间充质干细胞（hMSC）的特点是使用显微镜，免疫表型，其分化为脂肪细胞，软骨细胞和骨细胞的能力。结果：实验小鼠（诱导的肾小球肾炎和输注的hMSC）的肾脏中存在抗人CD 105+细胞，抗人结蛋白（系膜细胞的标志物）也呈阳性。免疫荧光检测显示，肾小球内抗人结蛋白阳性细胞处于增殖期，抗人Ki-67阳性。结论：肾小球损伤后，肾小球内hMSC在体内分化为系膜细胞。
Aims: Adult human bone marrow contains a population of mesenchymal stem cells (MSC) that contributes to the regeneration of tissues such as bone, cartilage, muscle, tendon, and fat. In recent years, it has been shown that functional stem cells exist in the adult bone marrow, and they can contribute to renal remodelling or reconstitution of injured renal glomeruli, especially mesangial cells. The purpose of this study is to examine the ability of MSC isolated from human bone marrow to differentiate into mesangial cells in glomerular injured athymic mice.Methods: MSC were isolated from human bone marrow mononuclear cells based on plastic adherent properties and expanded invitro in the culture medium. Human mesenchymal stem cells (hMSC) were characterised using microscopy, immunophenotyping, and their ability to differentiate into adipocytes, chondrocytes, and osteocytes. hMSC were then injected into athymic mice, which had induced glomerulonephropathy (GN).Results: Test mice (induced GN and infused hMSC) were shown to have anti-human CD105+ cells present in the kidneys and were also positive to anti-human desmin, a marker for mesangial cells. Furthermore, immunofluorescence assays also demonstrated that anti-human desmin+ cells in the glomeruli of these test mice were in the proliferation stage, being positive to anti-human Ki-67.Conclusions: These findings indicate that hMSC found in renal glomeruli differentiated into mesangial cells invivo after glomerular injury occurred.