A STUDY OF LIGHT INTENSITY, PERIODICITY, AND WAVELENGTH ON ZOOSPORE PRODUCTION BY PROTOSIPHON BOTRYOIDES KLEBS 1 2
A STUDY OF LIGHT INTENSITY, PERIODICITY, AND WAVELENGTH ON ZOOSPORE PRODUCTION BY PROTOSIPHON BOTRYOIDES KLEBS 1 2
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光强、周期性和波长对原管葡萄孢 KLEBS 游动孢子产生的研究 1 2
DOI：
10.1111/j.1529-8817.1968.tb04708.x
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发表时间：
1968
影响因子：
2.9
通讯作者：
J. O'Kelley
中科院分区：
文献类型：
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作者：
Jennie P. Durant;Larry Spratling;J. O'Kelley
当将成熟的原管管藻细胞置于黑暗中时，游动孢子的产生在27 ℃下比在22或15 ℃下更广泛，并且在更短的时间内完成。冷白色荧光（西尔瓦尼亚）光在辐射强度为0.6±103 ergs/cm 2-sec时可测量地抑制该过程;在14±103 ergs/cm 2-sec时，抑制完成率为96%。对于先前在重复的12 - 12小时光照-黑暗循环下生长的成熟细胞，在22 ℃下约2小时的黑暗期允许细胞分裂进行到一个阶段，使得再光照不会抑制游动孢子的继续发育。与黑暗相比，402至约-494 nm的单色光抑制游动孢子形成;最大抑制在432 - 461 nm处。与此相反，单色光从522至726 nm刺激游动孢子的形成相对于黑暗。使用以下方案获得同步游动孢子生产：（A）12小时冷-白色与12小时黄色交替，（B）12小时冷-白色与12小时蓝色交替。在方案A下，同步游动孢子释放（在同步生产之后）发生在接近黄色照射期结束时，而在方案B下，其发生在接近冷白色照射期结束时。这在光过程和可能的光感受器方面的意义进行了讨论。
When mature Protosiphon cells were placed in darkness, zoospore production was more extensive and was completed in a shorter time at a temperature of 27 C than at 22 or 15 C. Cool‐white fluorescent (Sylvania) light inhibited the process measurably at a radiation intensity of 0.6±103 ergsjcm2‐sec; inhibition was 96% complete at 14±103 ergs/cm2‐sec. For mature cells previously grown under repeated 12‐12 hr light‐dark cycles, a dark period of approximately 2 hr at 22 C allowed cell division to proceed to a stage such that reillumination did not inhibit continued development of zoospores. Monochromatic light from 402 to approximately ‐494 nm, as compared to darkness, inhibited zoospore formation; maximal inhibition was at 432‐461 nm. In contrast, monochromatic light from 522 to 726 nm stimulated zoospore formation relative to darkness. Synchronous zoospore production was obtained using the following regimes: (A) 12 hr cool‐white alternated with 12 hr yellow, (B) 12 hr cool‐white alternated with 12 hr blue. Under regime A synchronous zoospore release (following synchronous production) occurred near the end of the yellow irradiation period, while under regime B it occurred near the end of the cool‐white irradiation period. The significance of this in terms of photoprocesses and possible photoreceptors is discussed.