Transducer-Like Protein in Campylobacter jejuni With a Role in Mediating Chemotaxis to Iron and Phosphate.
Transducer-Like Protein in Campylobacter jejuni With a Role in Mediating Chemotaxis to Iron and Phosphate.
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DOI：
10.3389/fmicb.2018.02674
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发表时间：
2018
影响因子：
5.2
通讯作者：
Rajashekara G
中科院分区：
文献类型：
--
作者：
Chandrashekhar K;Srivastava V;Hwang S;Jeon B;Ryu S;Rajashekara G
趋化作用介导的运动使空肠弯曲菌能够在复杂的环境梯度中航行并在不同的生态位中定居。C.已知空肠具有几种甲基接受趋化蛋白（MCP），也称为转导子样蛋白（Tlps）。虽然一些TLPs在趋化性中的作用已经被确定，但它们在毒力中的调节和作用仍然不是很清楚。在此，我们研究了Tlp 2对C.空肠趋化性、应激存活和鸡胃肠道定植。Δ tlp 2缺失突变体对天冬氨酸、丙酮酸、无机磷酸盐（Pi）和铁（FeSO 4）的趋化性降低。用启动子融合报告基因测定对tlp 2的转录分析显示，tlp 2启动子（Ptlp 2）由亚铁（Fe 2+）和三价铁（Fe 3+）形式的Pi和铁诱导。使用重叠引物的RT-PCR分析表明，phoX基因，直接位于下游的tlp 2，与tlp 2共转录。在tlp 2起始密码子上游53 bp处鉴定了转录起始位点。Δ tlp 2突变体在鸡胃肠道中的定植减少。总的来说，我们的研究结果表明，tlp 2在C.空肠致病和在鸡宿主中的定殖，并且其表达受铁调节。
Chemotaxis-mediated motility enables Campylobacter jejuni to navigate through complex environmental gradients and colonize diverse niches. C. jejuni is known to possess several methyl accepting chemotaxis proteins (MCPs), also called transducer-like proteins (Tlps). While the role of some of the Tlps in chemotaxis has been identified, their regulation and role in virulence is still not very clear. Here, we investigated the contribution of Tlp2 to C. jejuni chemotaxis, stress survival and colonization of the chicken gastrointestinal tract. The Δtlp2 deletion mutant showed decreased chemotaxis toward aspartate, pyruvate, inorganic phosphate (Pi), and iron (FeSO4). Transcriptional analysis of tlp2 with a promoter fusion reporter assay revealed that the tlp2 promoter (Ptlp2) was induced by Pi and iron, both in the ferrous (Fe2+) and ferric form (Fe3+). RT-PCR analysis using overlapping primers indicated that the phoX gene, located immediately downstream of tlp2, is co-transcribed with tlp2. A transcription start site was identified at 53 bp upstream of the tlp2 start codon. The Δtlp2 mutant showed decreased colonization of the chicken gastrointestinal tract. Collectively, our findings revealed that the tlp2 plays a role in C. jejuni pathogenesis and colonization in the chicken host and its expression is regulated by iron.
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影响因子：
3.7
作者：
Drozd M;Gangaiah D;Liu Z;Rajashekara G
通讯作者：
Rajashekara G
影响因子：
4.2
作者：
Grabowska AD;Wandel MP;Łasica AM;Nesteruk M;Roszczenko P;Wyszyńska A;Godlewska R;Jagusztyn-Krynicka EK
通讯作者：
Jagusztyn-Krynicka EK
DOI：
10.1073/pnas.0907468107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Harris, H. W.;El-Naggar, M. Y.;Nealson, K. H.
通讯作者：
Nealson, K. H.
影响因子：
4.4
作者：
Gangaiah, Dharanesh;Kassem, Issmat I.;Rajashekara, Gireesh
通讯作者：
Rajashekara, Gireesh
影响因子：
3.6
作者：
Hartley-Tassell, Lauren E.;Shewell, Lucy K.;Korolik, Victoria
通讯作者：
Korolik, Victoria