Cell dynamics during somite boundary formation revealed by time-lapse analysis
Cell dynamics during somite boundary formation revealed by time-lapse analysis
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DOI：
10.1126/science.1075544
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发表时间：
2002-11-01
期刊：
影响因子：
56.9
通讯作者：
Fraser, SE
中科院分区：
文献类型：
--
作者：
Kulesa, PM;Fraser, SE
我们对活体鸡胚胎的体节分割进行了跟踪，发现成型过程不是简单的周期性切片组织块，而是更精心设计的分离，其中体节从节段板上拉出。细胞跨过假定的体节边界，并违反被认为与体节边界位置相关的基因表达边界。类似地，当细胞离开结节时，它们似乎没有预先分配给给定的体节。这些结果提供了体节形成的详细图像，并为将基因表达与细胞运动联系起来提供了时空框架。
We follow somite segmentation in living chick embryos and find that the shaping process is not simple periodic slicing of tissue blocks but much more carefully choreographed separation in which the somite pulls apart from the segmental plate. Cells move across the presumptive somite boundary and violate gene expression boundaries thought to correlate with the site of the somite boundary. Similarly, cells do not appear to be preassigned to given somite as they leave the node. The results offer detailed picture of somite shaping and provide spatiotemporal framework for linking gene expression with cell movements.