FULL LENGTH CDNA STRUCTURE AND DEDUCED AMINO-ACID SEQUENCE OF HUMAN 3-BETA-HYDROXY-5-ENE STEROID DEHYDROGENASE
FULL LENGTH CDNA STRUCTURE AND DEDUCED AMINO-ACID SEQUENCE OF HUMAN 3-BETA-HYDROXY-5-ENE STEROID DEHYDROGENASE
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DOI：
10.1210/mend-3-8-1310
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发表时间：
1989-08-01
影响因子：
--
通讯作者：
LABRIE, F
中科院分区：
文献类型：
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作者：
THE, VL;LACHANCE, Y;LABRIE, F
针对3 β-从人胎盘分离的羟基类固醇脱氢酶用于筛选来自相同组织的Gt 11表达cDNA文库。从cDNA序列推导的蛋白质含有372个氨基酸，计算的分子量为42，216。从3.beta开始-羟基类固醇脱氢酶是催化所有类型的激素类固醇形成的酶，编码该酶的cDNA的可用性为详细研究肾上腺、性腺以及外周组织中调节该关键酶的表达和活性的因素开辟了新的可能性。
Polyclonal antibodies raised against 3.beta.-hydroxysteroid dehydrogenase isolated from human placenta were used to screen a .lambda.gt11 expression cDNA library from the same tissue. The protein deduced from cDNA sequences contains 372 amino acids with a calculated mol wt of 42,216. Since 3.beta.-hydroxysteroid dehydrogenase is the enzyme catalyzing the formation of all classes of hormonal steroids, the availability of cDNA encoding this enzyme opens new possibilities for a detailed investigation of the factors regulating the expression and activity of this crucial enzyme in adrenal, gonadal as well as peripheral tissues.