Rapid colorimetric and fluorescence identification of Pinelliae Rhizoma and adulterate Rhizoma Typhonii Flagelliformis using direct-LAMP assay
Rapid colorimetric and fluorescence identification of Pinelliae Rhizoma and adulterate Rhizoma Typhonii Flagelliformis using direct-LAMP assay
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DOI：
10.1016/j.foodchem.2023.137840
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发表时间：
2023-10-31
期刊：
影响因子：
8.8
通讯作者：
Chen，Rong
中科院分区：
文献类型：
--
作者：
Li，Huilin;Cui，Jiaqi;Chen，Rong
半夏因其对咳嗽、失眠和炎症的药用价值而闻名，经常被用于植物膳食补充剂和草药产品。然而，由于需求很高，存在掺假的风险。介绍了一种快速比色技术--直接环介导恒温扩增(Direct-LAMP)技术，用于区分半夏及其主要混伪品香附。该方法由DNA免提取直接裂解、等温扩增和快速显色三部分组成。直接裂解过程允许在2分钟内释放基因组DNA。该等温扩增反应具有良好的分析性能，可检测到低至0.1微克的半夏和0.08微克的香椿，同时在较宽的范围内表现出良好的线性关系，适合于定量分析。对于这两种不同的植物材料，使用了两种不同的显色剂，导致了双重颜色的变化，以吸引人的视觉检测。所开发的直接灯也在48个实际样品中得到了成功的验证。
Pinelliae Rhizoma, renowned for its medicinal benefits against cough, insomnia and inflammation, is frequently utilized in botanical dietary supplements and herbal products. However, due to high demand, there is a risk of adulteration. This study introduced Direct-Loop-Mediated Isothermal Amplification (Direct-LAMP), a rapid colorimetric technique, for distinguishingPinelliae Rhizomafrom its primary adulterant,Rhizoma Typhonii Flagelliformis. This method comprises three parts: DNA extraction-free direct lysis, isothermal amplification and prompt color development. The direct lysis process allows for release of genomic DNA within 2 min. The isothermal amplification reaction exhibits excellent analytical performance, detectingPinelliae Rhizomaat levels as low as 0.1 pg andRhizoma Typhonii Flagelliformisat 0.08 ng, while also demonstrating good linearity across a wide range, making it suitable for quantitative analysis. Two different color reagents are employed for these two different plant materials, resulting in dual-color changes for captivating visual detection. The developed direct-LAMP was also successfully validated across 48 actual samples.