Formation and Closure of Microchannels in Skin Following Microporation
Formation and Closure of Microchannels in Skin Following Microporation
复制标题
DOI：
10.1007/s11095-010-0122-x
复制
发表时间：
2011-01-01
影响因子：
3.7
通讯作者：
Banga, Ajay K.
中科院分区：
文献类型：
--
作者：
Kalluri, Haripriya;Banga, Ajay K.
目的研究微针在无毛大鼠皮肤中形成的微通道的特征，并探讨其在不同闭合条件下的闭合情况。方法采用扫描电镜对麦芽糖微针进行表征。使用各种technology.Results显微镜成像表明麦芽糖微针的平均长度为559 +/- 14 μ m和尖端半径为4 μ m的金字塔几何形状的微通道创建和他们的封闭时，暴露于不同的条件进行了调查。在插入皮肤后，他们创建了平均表面直径为60 μ m，平均深度为160 +/- 20 μ m的微通道，通过组织切片和共聚焦显微镜观察。当暴露于环境时，皮肤在3-4小时内恢复其屏障功能，并且微通道在穿孔后15小时内关闭。然而，闭塞时，微通道保持开放长达72小时，在体内，观察到的钙黄绿素成像，经表皮水分损失测量和亚甲蓝staining.Conclusion麦芽糖微针穿透角质层屏障，并创建在皮肤中的微通道完全关闭后15小时内穿孔。然而，在闭塞条件下，屏障恢复可以在体内延迟长达72小时。
Purpose To characterize the microchannels created in hairless rat skin by microneedles and investigate their closure following exposure to different occlusive conditions.Methods Maltose microneedles were characterized by scanning electron microscopy. The microchannels created and their closure when exposed to different conditions was investigated using a variety of techniques.Results Microscopic imaging indicates a pyramidal geometry of maltose microneedles with an average length of 559 +/- 14 mu m and tip radius of 4 mu m. Upon insertion into skin, they created microchannels with an average surface diameter of 60 mu m and an average depth of 160 +/- 20 mu m as observed by histological sectioning and confocal microscopy. Skin recovers its barrier function within 3-4 hrs, and microchannels closed within 15 hrs of poration when exposed to environment. However, when occluded, the microchannels remained open for up to 72 hrs in vivo, as observed by calcein imaging, transepidermal water loss measurements and methylene blue staining.Conclusion Maltose microneedles penetrated the stratum corneum barrier and created microchannels in skin which completely close within 15 hrs after poration. However, under occluded conditions, barrier recovery can be delayed for up to 72 hrs in vivo.