Non-invasive mapping of glutathione levels in mouse brains by in vivo electron paramagnetic resonance (EPR) imaging: Applied to a kindling mouse model
Non-invasive mapping of glutathione levels in mouse brains by in vivo electron paramagnetic resonance (EPR) imaging: Applied to a kindling mouse model
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DOI：
10.1016/j.neulet.2018.10.001
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发表时间：
2019-01-18
影响因子：
2.5
通讯作者：
Fujii, Hirotada G.
中科院分区：
文献类型：
--
作者：
Emoto, Miho C.;Sato-Akaba, Hideo;Fujii, Hirotada G.
谷胱甘肽（GSH）是一种重要的抗氧化剂，可以在氧化应激下保护细胞。因此，需要一种非侵入性的方法来测量和绘制活动物体内谷胱甘肽的分布。为了对GSH水平在脑特定区域的分布进行成像，提出了一种利用电子顺磁共振（EPR）与氮氧化物成像探针成像的新方法。利用体内EPR成像仪测量的大脑中氮氧化物还原率与体外生化试验测量的大脑GSH水平之间的线性关系，成功地获得了基于像素的脑GSH水平映射。该方法应用于戊四唑（PTZ）诱导的小鼠模型，观察癫痫发作后特定脑区谷胱甘肽水平的变化。获得的脑谷胱甘肽水平图清楚地表明，与对照小鼠相比，海马区周围的谷胱甘肽水平下降。
Glutathione (GSH) is an important antioxidant that can protect cells under oxidative stress. Thus, a non-invasive method to measure and map the distribution of GSH in live animals is needed. To image the distribution of GSH levels in specific brain regions, a new method using electron paramagnetic resonance (EPR) imaging with a nitroxide imaging probe was developed. Pixel-based mapping of brain GSH levels was successfully obtained by using the linear relationship between reduction rates for nitroxides in brains, measured by an in vivo EPR imager, and brain GSH levels, measured by an in vitro biochemical assay. The newly developed method was applied to a kindling mouse model induced with pentylenetetrazole (PTZ) to visualize changes in GSH levels in specific brain regions after seizure. The obtained map of brain GSH levels clearly indicated decreased GSH levels around the hippocampal region compared to control mice.