Cofolding organizes alfalfa mosaic virus RNA and coat protein for replication.
Cofolding organizes alfalfa mosaic virus RNA and coat protein for replication.
复制标题
共折叠组织苜蓿花叶病毒 RNA 和外壳蛋白进行复制。
DOI：
10.1126/science.1103399
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Gehrke,Lee
中科院分区：
文献类型：
--
作者：
Guogas,LauraM;Filman,DavidJ;Hogle,JamesM;Gehrke,Lee
只有当病毒外壳蛋白与RNA的3′端结合时，Alkalimosaic病毒基因组RNA才具有感染性。苜蓿花叶病毒RNA-肽复合物的晶体结构揭示了保守的AUGC重复序列和Pro-Thr-x-Arg-Ser-x-x-Tyr外壳蛋白氨基酸在相互作用时共折叠。交替的AUGC残基具有相反的方向，并且它们在不同的相邻双链体中碱基配对。通过腺嘌呤-鸟嘌呤相互作用稳定的局部RNA骨架反转将腺苷和鸟嘌呤以相反的顺序放置在双链体中。结果表明，一个统一的，有组织的3′构象，类似于病毒RNA与转移RNA样末端，可能是复制所必需的。
Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3′ termini. The crystal structure of an alfalfa mosaic virus RNA-peptide complex reveals that conserved AUGC repeats and Pro-Thr-x-Arg-Ser-x-x-Tyr coat protein amino acids cofold upon interacting. Alternating AUGC residues have opposite orientation, and they base pair in different adjacent duplexes. Localized RNA backbone reversals stabilized by arginine-guanine interactions place the adenosines and guanines in reverse order in the duplex. The results suggest that a uniform, organized 3′ conformation, similar to that found on viral RNAs with transfer RNA–like ends, may be essential for replication.