D3 dopamine receptor activates phospholipase D through a pertussis toxin-insensitive pathway
D3 dopamine receptor activates phospholipase D through a pertussis toxin-insensitive pathway
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DOI：
10.1016/j.neulet.2004.08.033
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发表时间：
2004-11-16
影响因子：
2.5
通讯作者：
Senogles, SE
中科院分区：
文献类型：
--
作者：
Everett, PB;Senogles, SE
Within the dopamine receptor family, the D-3 dopamine receptor's function remains inadequately described. The D-3 receptor has been shown to couple to inhibition of adenylyl cyclase, stimulation of mitogenesis, and regulation of K+ and Ca2+ currents, all in a pertussis toxin (PTX)-sensitive manner. Here we report D-3 receptor activation of the phospholipase D (PLD) enzyme in HEK 293 cells heterologously expressing the human D-3 receptor. Activation by agonist is dose dependent and displays the pharmacology expected of the D-3 receptor. The D-3 receptor specific antagonists AJ-76 and U99194A ablated the increase in activity by the preferring D-3 agonist (+)7-OH DPAT. In addition, the D-3 receptor-mediated activation of PLD is not mediated by G-proteins of the G(i)/G(o) family, as pretreatment with PTX had no effect. PLD activation is a novel finding for the D-3 receptor, and is the first example of an effector system where D-3 signals without G(i)/G(o) protein intermediates. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
