Enhancement of local bone formation on titanium implants in osteoporotic rats by biomimetic multilayered structures containing parathyroid hormone (PTH)-related protein
Enhancement of local bone formation on titanium implants in osteoporotic rats by biomimetic multilayered structures containing parathyroid hormone (PTH)-related protein
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含甲状旁腺激素（PTH）相关蛋白的仿生多层结构增强骨质疏松大鼠钛种植体局部骨形成
DOI：
10.1088/1748-605x/ab7b3d
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发表时间：
2020-07-01
影响因子：
4
通讯作者：
Shen, Liyan
中科院分区：
文献类型：
--
作者：
Tang, Jiahao;Yan, Deyi;Shen, Liyan
骨质疏松症是一种严重的健康问题，导致骨骼脆弱和随之而来的骨折。钛（Ti）植入物用于骨质疏松性骨折患者，由于骨量和强度下降，容易失败。因此，制造具有成骨特性的种植体以改善种植体的骨整合是至关重要的。为了提高骨质疏松患者钛植入物的长期存活率，采用分层（LBL）电组装技术在钛植入物表面沉积含有甲状旁腺激素（PTH）相关蛋白（PTHrP）的透明质酸/epsilon-聚赖氨酸多层膜。利用具有高成骨分化潜力的小鼠成骨前细胞系MC3T3-E1，体外评价Ti-LBL-PTHrPin的成骨诱导作用。此外，在Sprague Dawley大鼠股骨髓内植入中，对Ti （Ti- lbl - pthrp）植入物的性能进行了体内评价。Ti-LBL-PTHrP种植体调节装载PTHrP的释放，在种植早期增加骨形成。体外结果显示，与未包被Ti组相比，Ti- lbl - pthrp组细胞增殖不明显，但碱性磷酸酶活性和成骨相关蛋白表达水平较高（p< 0.05）。此外，体内显微ct和组织学分析表明，植入后14 d， Ti-LBL-PTHrP植入物可显著促进骨质疏松大鼠新骨的形成和重塑。总的来说，这项研究为治疗骨质疏松症的生物功能钛植入物的制造建立了一个深刻而直接的方法。
Osteoporosis is a severe health problem causing bone fragility and consequent fracture. Titanium (Ti) implants, used in patients with osteoporotic fractures, are prone to failure because of the decreased bone mass and strength. Therefore, it is of utmost importance to fabricate implants possessing osteogenic properties to improve implant osseointegration. To improve the long-term survival rate of Ti implants in osteoporotic patients, hyaluronic acid/epsilon-polylysine multilayers containing the parathyroid hormone (PTH)-related protein (PTHrP) were deposited on Ti implants by a layer-by-layer (LBL) electro assembly technique. The murine pre-osteoblast cell line MC3T3-E1, possessing a high potential of osteoblast differentiation, was used to evaluate the osteo-inductive effects of Ti-LBL-PTHrPin vitro. In addition, the performance of the Ti (Ti-LBL-PTHrP) implant was evaluatedin vivoin a femoral intramedullary implantation in Sprague Dawley rats. The Ti-LBL-PTHrP implant regulated the release of the loaded PTHrP to increase bone formation in the early stage of implantation. Thein vitroresults revealed that cells on Ti-LBL-PTHrP did not show any evident proliferation, but a high level of alkaline phosphatase activity and osteoblast-related protein expression was found, compared to the uncoated Ti group (p< 0.05). In addition,in vivomicro-CT and histological analysis demonstrated that the Ti-LBL-PTHrP implants could significantly promote the formation and remodeling of new bone in osteoporotic rats at 14 d after implantation. Overall, this study established a profound and straightforward methodology for the manufacture of biofunctional Ti implants for the treatment of osteoporosis.