Ultrasound biomicroscopy of the rat eye: effects of cholinergic and anticholinergic agents.
Ultrasound biomicroscopy of the rat eye: effects of cholinergic and anticholinergic agents.
复制标题
大鼠眼的超声生物显微镜检查：胆碱能和抗胆碱能药物的作用。
DOI：
10.1007/s00417-004-1061-1
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Danias,John
中科院分区：
文献类型：
--
作者：
Nissirios,Nicholas;Ramos-Esteban,Jerome;Danias,John
背景在过去的几年里，大鼠作为青光眼研究的动物模型已经获得了重视。然而，迄今为止，尚未有关于大鼠房角结构和药物对房角解剖结构影响的系统研究报告。我们调查了正常大鼠眼前段解剖在体内使用超声生物显微镜（UBM）和确定的影响，两个胆碱能和抗胆碱能药物对角structures. Methods 14眼7个月大的雌性Wistar大鼠成像使用超声生物显微镜和修改后的eyecup。测量前房深度（ACD）、小梁-虹膜角（TIA）、瞳孔缘最厚点虹膜厚度（IT）、开角距离（AOD）（在距巩膜棘200 μm处测量角膜后表面与虹膜前表面之间的距离）、角膜厚度（CT）和虹膜-悬韧带距离（IZD）。在滴注1滴1%环喷托酯后30分钟和滴注1%毛果芸香碱后48小时后30分钟重复成像。获得相同的测量值，并与基线value.ResultsBaseline记录的所有参数的值没有显着不同的对侧眼睛。滴注毛果芸香碱或环喷托酯后，ACD是唯一一个相对于基线无显著变化的参数。而TIA、AOD、IZD、IT在三组间差异有统计学意义。事后分析（Bonferroni检验）显示TIA和AOD在所有三组眼睛之间存在差异。毛果芸香碱治疗组与其他两组的IZD和IT之间也存在差异。毛果芸香碱治疗组与基线CT测量值之间的差异非常小，这是由于前一组测量值的方差为零所致。虽然毛果芸香碱和环喷托酯引起的角度狭窄，检查超声图像显示差异的影响。毛果芸香碱造成了“瞳孔块样”的图片，而环喷托酯造成拥挤的虹膜基地在angle.ConclusionsBaseline正常大鼠前房解剖的特点建立。环喷托酯和毛果芸香碱均通过不同的机制引起大鼠眼睛角度变窄。
BackgroundOver the past few years the rat has gained prominence as an animal model for the study of glaucoma. However, no systematic study of the angle structures and the effects of medications on angle anatomy in the rat has been reported to date. We investigated the normal rat anterior segment anatomy in vivo using ultrasound biomicroscopy (UBM) and determined the effect of both cholinergic and anticholinergic medications on angle structures.MethodsFourteen eyes of seven 2-month-old female Wistar rats were imaged using an ultrasound biomicroscope and a modified eyecup. Baseline measurements of the anterior chamber depth (ACD), trabecular-iris angle (TIA), iris thickness at the thickest point near the pupillary margin (IT), angle-opening distance (AOD) (distance between the posterior corneal surface and anterior iris surface measured at 200 μm from the scleral spur), corneal thickness (CT) and irido-zonular distance (IZD) were obtained. Imaging was repeated 30 min after instillation of one drop of cyclopentolate 1% and 48 h later 30 min after pilocarpine 1% instillation. The same measurements were obtained and compared to baseline values.ResultsBaseline values for all parameters recorded were not significantly different among contralateral eyes. After instillation of either pilocarpine or cyclopentolate, ACD was the only parameter that did not change significantly from baseline. In contrast, TIA, AOD, IZD, and IT were significantly different among the three groups. Post-hoc analysis (Bonferroni test) revealed differences among all three groups of eyes for TIA and AOD. A difference was also found between the pilocarpine-treated group and the other two groups for IZD and IT. A very small difference detected between the pilocarpine-treated group and the baseline measurements for CT was caused by the zero variance of measurements in the former group. Although both pilocarpine and cyclopentolate induced angle narrowing, inspection of the ultrasonic images revealed a differential effect. Pilocarpine caused a “pupillary block-like” picture, while cyclopentolate caused crowding of the iris base in the angle.ConclusionsBaseline characteristics of the normal rat anterior chamber anatomy were established. Both cyclopentolate and pilocarpine cause angle narrowing in the rat eye, by different mechanisms.