Involvement of NAD(P)H:Quinone Oxidoreductase 1 and Superoxide Dismutase Polymorphisms in Ulcerative Colitis
Involvement of NAD(P)H:Quinone Oxidoreductase 1 and Superoxide Dismutase Polymorphisms in Ulcerative Colitis
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DOI：
10.1089/dna.2009.0877
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发表时间：
2009-12-01
影响因子：
3.1
通讯作者：
Watanabe, Masatoshi
中科院分区：
文献类型：
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作者：
Kosaka, Toshihito;Yoshino, Junji;Watanabe, Masatoshi
炎症性肠病是一种多因素疾病。氧化应激被认为是炎症性肠病的病因之一。超氧化物歧化酶 (SOD2) 和 NAD(P)H:醌氧化还原酶 1 (NQO1) 基因与炎症和氧化应激有关。本病例对照研究的目的是确定 134 名溃疡性结肠炎 (UC) 患者和 125 名健康对照者的基因多态性（NQO1 C609T 和 SOD2 Ala-9Val）是否与 UC 风险相关并影响临床特征。通过聚合酶链反应-限制性片段长度多态性和直接测序来检查这些多态性。在显示类固醇抵抗的患者中，NQO1 T/T 基因型的数量显着高于其他基因型（比值比 9.45，95% 置信区间 2.46-41.6，p = 0.002）。在 UC 发病年龄为 20 岁或以下的患者中，SOD2 T/T 基因型的患者多于其他基因型的患者（比值比 6.46，95% 置信区间 0.82-51.0）。这些多态性与 UC 风险之间没有明显的关联。 NQO1 C609T 多态性可能影响 UC 患者的类固醇抵抗，而 SOD2 Ala-9Val 多态性可能影响 UC 的发病年龄。氧化应激可能影响 UC 的临床特征。
Inflammatory bowel disease is a multifactorial disease. Oxidative stress has been thought to be one of etiologic factor for inflammatory bowel disease. The genes superoxide dismutase (SOD2) and NAD(P)H:quinone oxidoreductase 1 (NQO1) are involved in inflammation and oxidative stress. The purpose of the present case-control study with 134 patients with ulcerative colitis (UC) and 125 healthy controls was to determine whether polymorphisms of these genes, the NQO1 C609T and the SOD2 Ala-9Val, are associated with the risk of UC and influence the clinical characteristics. These polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphisms and direct sequencing. In patients showing steroid resistance, the number with the NQO1 T/T genotype was significantly higher than other genotypes (odds ratio 9.45, 95% confidence interval 2.46-41.6, p = 0.002). In the patients whose onset of UC was age 20 years or younger, more patients had SOD2 T/T genotype than the other genotypes (odds ratio 6.46, 95% confidence interval 0.82-51.0). No association between these polymorphisms and UC risk was apparent. The NQO1 C609T polymorphism may influence steroid resistance of UC patients, while the SOD2 Ala-9Val polymorphism may influence age of onset of UC. Oxidative stress may influence the clinical features of UC.