Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6
Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6
复制标题
中国小麦优良品种小偃6号籽粒发育过程中白蛋白和球蛋白动态
DOI：
10.1016/j.jcs.2012.08.016
复制
发表时间：
2012-11-01
影响因子：
3.8
通讯作者：
Yan, Yueming
中科院分区：
文献类型：
--
作者：
Dong, Kun;Ge, Pei;Yan, Yueming
以小偃6号为材料，研究了小麦籽粒发育过程中清蛋白和球蛋白的蛋白质组动态。共210个差异表达的蛋白质点被识别的二维差异凝胶电泳（2D-DIGE）和146这些代表89个独特的蛋白质进行了MALDI-TOF质谱鉴定。在5个籽粒发育阶段观察到4种蛋白质表达模式。在鉴定的蛋白质中，超过80%是8种功能类别的酶，包括碳水化合物代谢（27%），蛋白质代谢（27%），应激/防御/解毒（11%），细胞代谢（6%），转录/翻译（4%），氮代谢（4%），光合作用（4%）和信号转导（1%）。对18个代表性基因的定量实时聚合酶链反应（qRT-PCR）分析表明，约三分之二的基因在转录和翻译阶段之间显示出不同的表达谱。(C)2012爱思唯尔有限公司保留所有权利。
Proteome Dynamics of albumins and globulins during grain development of elite Chinese wheat cultivar Xiaoyan 6 were studied. A total of 210 differentially expressed protein spots were recognized by two-dimensional differential gel electrophoresis (2D-DIGE) and 146 of these that represented 89 unique proteins were identified by MALDI-TOF mass spectrometry. Four protein expression patterns were observed across five grain developmental stages. Among the identified proteins, more than 80% were enzymes in eight functional categories, including carbohydrate metabolism (27%), protein metabolism (27%), stress/defense/detoxification (11%), cell metabolism (6%), transcription/translation (4%), nitrogen metabolism (4%), photosynthesis (4%), and signal transduction (1%). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 18 representative genes demonstrated that about two thirds of the genes displayed different expression profiles between the transcriptional and translational stages. (C) 2012 Elsevier Ltd. All rights reserved.