Qualitative aspects of sperm stock in males and females from Eupelmus orientalis and Dinarmus basalis (Hymenoptera: Chalcidoidea) as revealed by dual fluorescence
Qualitative aspects of sperm stock in males and females from Eupelmus orientalis and Dinarmus basalis (Hymenoptera: Chalcidoidea) as revealed by dual fluorescence
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DOI：
10.1046/j.1365-3032.2002.00274.x
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发表时间：
2002-06-01
影响因子：
1.5
通讯作者：
Chevrier, C
中科院分区：
文献类型：
--
作者：
Damiens, D;Bressac, C;Chevrier, C
精子群体的质量可以用生理学特征来描述，其繁殖力可以通过其活精子与非活精子的比率来预测。为提高人们对两种体外寄生膜翅目昆虫(膜翅目：真姬蜂Eupelmus orientalisCrawford(膜翅目：Eupelmidae)和金小蜂Dinarmus basalis Rondani(膜翅目：翼蜂科))生殖策略的认识，建立了用SYBR-14：碘丙酸双重荧光染色技术检测精子活力的方法。这项研究的目的是提供一种适用于两性的体外比较试验，以研究生殖过程不同阶段精子质量的演变。在两种压力测试(乙醇和Triton X-100)的基础上，两种物种都证实了碘化丙胺检测无活力精子(红色染色)的可靠性，但我们的研究也表明，必须针对每一种物种充分调整碘化丙胺的浓度。本实验还证实了东方龙虾和北欧龙虾雄性和雌性精子群体的生理异质性。在这两个物种中，精囊中40%的精子被发现是不能存活的。与东方刺蛾相比，从东方刺蛾精囊中估计的不能存活精子的数量与在受精囊中观察到的精子数量有很大的不同。从目前的结果来看，在两个物种的雌性受精囊中检测到的可存活精子数量被证明是产卵雌性受精的可靠预测因子。
The quality of a sperm population can be characterized physiologically and its fecundity predicted by its viable : non-viable sperm ratio. To improve the knowledge of reproductive strategies in two ectoparasitoid hymenopteran species, Eupelmus orientalis Crawford (Hymenoptera: Eupelmidae) and Dinarmus basalis Rondani (Hymenoptera: Pteromalidae), the assessment of sperm viability using the dual fluorescence staining procedure SYBR-14 : propidium iodide was developed. The aim of the study was to provide a comparative test in vitro applicable to both sexes to study the evolution of sperm quality at various stages of the reproductive processes. The reliability of propidium iodide to detect non-viable sperm (stained in red) was confirmed in both species on the basis of two stress tests (ethanol and Triton X-100) but our study also revealed that propidium iodide concentrations must be adequately adjusted for each single species. This experiment also demonstrated the physiological heterogeneity of sperm populations in E. orientalis and D. basalis males and females. In both species, 40% of the sperm in the seminal vesicles was found to be non-viable. By contrast with E. orientalis, the populations of non-viable sperm estimated from the seminal vesicles of D. basalis were found to be strongly different from those observed in the spermatheca. From the present results, the population of viable sperm detected in the spermatheca of females from both species proved a reliable predictor of fertilization achieved in ovipositing females.