Analysis of electrophysiological properties and responses of neutrophils.
Analysis of electrophysiological properties and responses of neutrophils.
复制标题
分析中性粒细胞的电生理特性和反应。
DOI：
10.1007/978-1-59745-467-4_11
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
DeCoursey,ThomasE
中科院分区：
文献类型：
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作者：
Morgan,Deri;DeCoursey,ThomasE
在过去的十年中，膜片钳技术越来越多地应用于中性粒细胞和嗜酸性粒细胞。这些电生理学研究的主要目的是阐明吞噬细胞呼吸爆发的机制。NADPH氧化酶活性定义了粒细胞中的呼吸爆发，它是产电的，因为来自NADPH的电子被转运穿过细胞膜，在细胞膜上它们还原氧形成超氧阴离子（O2−）。如果电荷运动没有被质子流出所平衡，电子的这种通过包括电流，该电流将迅速使膜去质子化。膜片钳技术能够同时记录NADPH氧化酶产生的电子电流和通过密切相关的H+通道的H+通量。越来越多的证据表明，其他离子通道可能发挥至关重要的作用，在脱粒，吞噬作用，和趋化性，突出的重要性，电生理学研究，以推进粒细胞功能的知识。膜片钳技术存在几种配置。每一种都有优点和局限性，在这里讨论。如果不了解离子通道的基本性质，就无法对离子通道进行有意义的测量。我们描述的类型的测量是必要的，以表征一个特定的离子通道。
The past decade has seen increasing use of the patch clamp technique on neutrophils and eosinophils. The main goal of these electrophysiological studies has been to elucidate the mechanisms underlying the phagocyte respiratory burst. NADPH oxidase activity, which defines the respiratory burst in granulocytes, is electrogenic because electrons from NADPH are transported across the cell membrane, where they reduce oxygen to form superoxide anion (O2−). This passage of electrons comprises an electrical current that would rapidly depolarize the membrane if the charge movement were not balanced by proton efflux. The patch clamp technique enables simultaneous recording of NADPH oxidase-generated electron current and H+flux through the closely related H+channel. Increasing evidence suggests that other ion channels may play crucial roles in degranulation, phagocytosis, and chemotaxis, highlighting the importance of electrophysiological studies to advance knowledge of granulocyte function. Several configurations of the patch clamp technique exist. Each has advantages and limitations that are discussed here. Meaningful measurements of ion channels cannot be achieved without an understanding of their fundamental properties. We describe the types of measurements that are necessary to characterize a particular ion channel.