Carbon dot cluster as an efficient "off-on" fluorescent probe to detect Au(III) and glutathione
Carbon dot cluster as an efficient "off-on" fluorescent probe to detect Au(III) and glutathione
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DOI：
10.1016/j.bios.2014.12.027
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发表时间：
2015-06-15
影响因子：
12.6
通讯作者：
Xi, Kai
中科院分区：
文献类型：
--
作者：
Gu, Jiangjiang;Hu, Donghua;Xi, Kai
本文首次报道了Au（III）修饰碳点簇（Au（III）/CDC）荧光“关-开”法检测谷胱甘肽。通过将Au（III）引入到发光碳点（CD）上，形成Au（III）/CDC复合物，有效地猝灭了CD的荧光，实现了“关闭”过程。该“关”过程用于检测Au（III），在21种金属离子中具有选择性，检测限为0.48 μ M（S/N=3）。溶液中的双硫醇可将Au（III）从配合物中除去，使CDC的荧光恢复，实现“开”过程。此过程是选择性的biothiols（尤其是谷胱甘肽）之间的多巴胺，多巴胺和氨基酸和检测限为2.02 μ M（S/N=3）。由于荧光恢复依赖于谷胱甘肽的浓度，Au（III）/CDC被用作荧光传感器用于检测溶液和细胞胞质溶胶中的谷胱甘肽。通过参考溶液中的荧光变化，评价有/无氧应激的细胞内谷胱甘肽。与商业化的方法相比，本研究所建立的Au（III）/CDC方法具有简便、快速、成本低等优点，可用于不同细胞状态下细胞内谷胱甘肽的测定。(C)2015 Elsevier B. V.版权所有。
In this paper, we reported for the first time that Au(III) decorated carbon dot cluster (Au(III)/CDC) was synthesized to detect glutathione through fluorescence "off-on" approach. The "off' process was realized by the introduction of Au(III) on luminescent carbon dots (CDs), which formed the complex of Au(III)/CDC and quenched the fluorescence of CDs efficiently. This "off" process was used to detect Au(III) with the selectivity among 21 metal ions and the limitation was 0.48 mu M (S/N=3). Au(III) could be removed from the complex by biothiol in the solution, which restored the fluorescence of CDC to achieve the "on" process. This process was selective for biothiols (especially for glutathione) among saccharides, dopamine and amino acids and the limit of detection was 2.02 mu M (S/N=3). Due to the dependence of the fluorescence restoration on the concentration of glutathione, Au(III)/CDC was applied as the fluorescence sensor for detection of glutathione in the solution and cellular cytosol. By referring to the fluorescence change in the solution, the intracellular glutathione with/without oxygen stress was evaluated. As compared with the commercial assay, our Au(III)/CDC based assay was simple, facile and low cost, which would be useful to measure intracellular glutathione at different cellular states. (C) 2015 Elsevier B.V. All rights reserved.