Phylogenetic analysis of members of the genus Rickettsia using the gene encoding the outer-membrane protein rOmpB (ompB)
Phylogenetic analysis of members of the genus Rickettsia using the gene encoding the outer-membrane protein rOmpB (ompB)
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DOI：
10.1099/00207713-50-4-1449
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发表时间：
2000-07-01
影响因子：
2.8
通讯作者：
Raoult, D
中科院分区：
文献类型：
--
作者：
Roux, V;Raoult, D
为了验证先前通过比较柠檬酸合成酶和Rompa基因序列(分别为GLTA和OmpA)而推断的系统发育分析，对24株立克次体的rOmpB基因(OmpB)进行了扩增和测序。ROmpB是一种高相对分子质量的外膜蛋白，免疫印迹交叉反应表明，大多数立克次体中都存在rOmpB。贝氏立克次体和加拿大立克次体均未获得扩增产物。对于其他立克次体，采用简约法、最大似然法和邻接法进行系统发育分析，得到了相同的组织结构。除包括非洲立克次体S和帕克立克次体外，所有节点都得到了很好的支持(>；86%的Bootstrap值)，这一分析证实了先前通过结合GLTA和OmpA的结果而建立的系统发育关系。根据系统发育数据，目前对立克次体的分类是不适当的，特别是将其分为斑疹伤寒和斑点热两个组，表型、基因和系统发育数据的结合将有助于定义一个多相分类，就像对其他细菌属所做的那样。
To confirm the phylogenetic analysis previously inferred by comparison of the citrate synthase and rOmpA gene sequences (gltA and ompA, respectively), the rOmpB gene (ompB) of 24 strains of the genus Rickettsia was amplified and sequenced. rOmpB is an outer-membrane protein of high molecular mass, the presence of which can be demonstrated in most rickettsiae by immunological cross-reactivity in Western blots. No PCR amplification was obtained with Rickettsia bellii or Rickettsia canadensis. For the other rickettsiae, phylogenetic analysis was inferred from the comparison of both the gene and derived protein sequences by using parsimony, maximum-likelihood and neighbour-joining methods which gave the same organization. All nodes were well supported (>86 % bootstrap values), except in the cluster including Rickettsia africae strain S and Rickettsia parkeri, and this analysis confirmed the previously established phylogeny obtained from combining results from gltA and ompA. Based on phylogenetic data, the current classification of the genus Rickettsia is inappropriate, specifically its division into two groups, typhus and spotted fever, integration of phenotypic, genotypic and phylogenetic data will contribute to the definition of a polyphasic taxonomy as has been done for other bacterial genera.