LATE LOW MAGNESIUM-INDUCED EPILEPTIFORM ACTIVITY IN RAT ENTORHINAL CORTEX SLICES BECOMES INSENSITIVE TO THE ANTICONVULSANT VALPROIC ACID
LATE LOW MAGNESIUM-INDUCED EPILEPTIFORM ACTIVITY IN RAT ENTORHINAL CORTEX SLICES BECOMES INSENSITIVE TO THE ANTICONVULSANT VALPROIC ACID
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DOI：
10.1016/0304-3940(90)90757-z
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发表时间：
1990-10-30
影响因子：
2.5
通讯作者：
HEINEMANN, U
中科院分区：
文献类型：
--
作者：
DREIER, JP;HEINEMANN, U
我们利用细胞外记录技术研究了低镁诱导的大鼠内嗅皮质/海马联合切片癫痫样活动的时间依赖性变化。在长时间的记录期间，CA3区会产生短暂的间歇放电，而活动没有任何重大变化，而内嗅皮层的初始强直性阵挛状事件可能随着时间而改变。我们经常观察到一种过渡到周期性强直活动状态，没有任何阵挛性后放电。或者，癫痫发作可以在实验的其余时间保持阵挛放电模式。抗惊厥药丙戊酸对这些不同的癫痫发作状态的影响并不相同。丙戊酸（VPA）可有效抑制内嗅皮质早期阵挛性强直性放电和CA3区间期样活动，但晚期复发性强直性发作放电状态不受药物影响。然而，它仍然对n -甲基- d -天冬氨酸（NMDA）受体拮抗剂2-氨基膦酸盐敏感。这些发现指出了癫痫引起的大鼠内嗅皮层神经元相互作用的变化。
We investigated time-dependent changes in low magnesium-induced epileptiform activity in combined rat entorhinal cortex/hippocampal slices with extracellular recording techniques. While in area CA3 short interictal discharges are generated without any major changes in activity during prolonged recording periods, initial tonic clonic ictaform events in the entorhinal cortex may change with time. We observed often a transition into a state of recurrent tonic activity without any clonic afterdischarges. Alternatively, seizures could stay in the clonic discharge mode for the rest of the experiment. These different seizure states were not equally affected by the anticonvulsant valproic acid. While the early clonic tonic discharges in the entorhinal cortex and the interictal like activity in area CA3 were effectively suppressed by valproic acid (VPA) the late recurrent tonic seizure discharge state was unaffected by the drug. It was, however, still sensitive to the N-methyl-D-aspartate (NMDA) receptor antagonist 2-aminophosphonovalerate. These findings point to seizure-induced changes in neuronal interaction in rat entorhinal cortex.