Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection.
Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection.
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DOI：
10.1021/acsbiomaterials.5b00449
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发表时间：
2016-03-14
影响因子：
5.8
通讯作者：
Shafiee H
中科院分区：
文献类型：
--
作者：
Safavieh M;Kanakasabapathy MK;Tarlan F;Ahmed MU;Zourob M;Asghar W;Shafiee H
快速、灵敏和选择性的病原体检测在传染病诊断和治疗监测中至关重要。目前可用的基于聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）的诊断测定耗时、复杂且相对昂贵，因此限制了它们在资源有限的环境中的实用性。环介导等温扩增（LAMP）技术已被广泛用于开发用于病原体检测和核酸分析的快速和灵敏的诊断测定，并为革命性的床旁分子诊断带来了巨大的希望。在这里，我们回顾了新的基于LAMP的芯片实验室（LAMP）诊断检测开发的病原体检测在过去几年。我们根据病原体检测的设计策略回顾了各种基于LAMP的平台，并讨论了仍在开发中并已在商业管道中的基于LAMP的平台。这篇综述旨在指导LAMP技术在分子诊断和基因组扩增的PCR平台中的使用。
Rapid, sensitive, and selective pathogen detection is of paramount importance in infectious disease diagnosis and treatment monitoring. Currently available diagnostic assays based on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are time-consuming, complex, and relatively expensive, thus limiting their utility in resource-limited settings. Loop-mediated isothermal amplification (LAMP) technique has been used extensively in the development of rapid and sensitive diagnostic assays for pathogen detection and nucleic acid analysis and hold great promise for revolutionizing point-of-care molecular diagnostics. Here, we review novel LAMP-based lab-on-a-chip (LOC) diagnostic assays developed for pathogen detection over the past several years. We review various LOC platforms based on their design strategies for pathogen detection and discuss LAMP-based platforms still in development and already in the commercial pipeline. This review is intended as a guide to the use of LAMP techniques in LOC platforms for molecular diagnostics and genomic amplifications.