EXPRESSION OF THE METASTATIC PHENOTYPE IN CELLS TRANSFECTED WITH HUMAN METASTATIC TUMOR DNA
EXPRESSION OF THE METASTATIC PHENOTYPE IN CELLS TRANSFECTED WITH HUMAN METASTATIC TUMOR DNA
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DOI：
10.1073/pnas.82.6.1726
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
WEINBERG, RA
中科院分区：
文献类型：
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作者：
BERNSTEIN, SC;WEINBERG, RA
NIH 3 T3小鼠成纤维细胞在被分离自EJ人膀胱癌细胞系的Ha-ras癌基因转化后形成非转移性纤维肉瘤，并皮下注射。接种到免疫活性NFS/NCr小鼠中。将来自人转移性肿瘤的DNA转染到这些Ha-ras转化体中，并且在s.c.接种。从该转移中分离DNA，并进行第二轮转移到Ha-ras转化的NIH 3 T3细胞中。将这些转染的培养物接种到小鼠中再次导致转移瘤的形成，这次是以更高的频率。对其中4个转移瘤的检查显示了所有4个转移瘤共有的离散人类DNA片段。转移表型可以通过DNA从细胞转移到细胞，并且与离散DNA区段的存在相关。该片段与myc癌基因或任何常见的ras肿瘤癌基因都不相同。
NIH 3T3 mouse fibroblasts form nonmetastasizing fibrosarcomas upon transformation by the Ha-ras oncogene isolated from the EJ human bladder carcinoma cell line and s.c. inoculation into immunocompetent NFS/NCr mice. DNA from a human metastatic tumor was transfected into these Ha-ras transformants, and one of the resulting colonies yielded a lung metastasis after s.c. inoculation. DNA was isolated from this metastasis and subjected to a 2nd round of transfer into Ha-ras-transformed NIH 3T3 cells. Inoculation of these transfected cultures into mice led once again to formation of metastases, this time at a higher frequency. Examination of four of the resulting metastases revealed discrete human DNA fragments that were common to all 4. The metastatic phenotype can be transferred via DNA from cell to cell and is associated with the presence of a discrete DNA segment. This segment is not identical to the myc oncogene or to any of the frequently detected ras tumor oncogenes.