Single particle analysis based on Zernike phase contrast transmission electron microscopy
Single particle analysis based on Zernike phase contrast transmission electron microscopy
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DOI：
10.1016/j.jsb.2007.10.015
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发表时间：
2008-02-01
影响因子：
3
通讯作者：
Nagayama, Kuniaki
中科院分区：
文献类型：
--
作者：
Danev, Radostin;Nagayama, Kuniaki
我们提出了第一个应用Zernike相衬透射电子显微镜的单粒子3D重建的蛋白质，使用GroEL伴侣蛋白作为测试样本。我们通过比较来自Zernike相衬成像的3D模型，与传统的欠焦相衬成像的模型，评估了该技术的性能。通过两种成像方法实现了相同的分辨率，约12埃。基于Zernike相衬数据的重建需要减少约30%的粒子。讨论了每种技术的优点和前景。(C)2007年爱思唯尔公司All rights reserved.
We present the first application of Zernike phase-contrast transmission electron microscopy to single-particle 3D reconstruction of a protein, using GroEL chaperonin as the test specimen. We evaluated the performance of the technique by comparing 3D models derived from Zernike phase contrast imaging, with models from conventional underfocus phase contrast imaging. The same resolution, about 12 angstrom, was achieved by both imaging methods. The reconstruction based on Zernike phase contrast data required about 30% fewer particles. The advantages and prospects of each technique are discussed. (C) 2007 Elsevier Inc. All rights reserved.