Photon-counting H33D detector for biological fluorescence imaging
Photon-counting H33D detector for biological fluorescence imaging
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DOI：
10.1016/j.nima.2006.05.155
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发表时间：
2006-11-01
影响因子：
1.4
通讯作者：
Weiss, S.
中科院分区：
文献类型：
--
作者：
Michalet, X.;Siegmund, O. H. W.;Weiss, S.
我们研制了一种用于荧光样品生物成像的光子计数高时空分辨率、高通量三维检测器（H33D）。该设计是基于一个25毫米直径的S20光电阴极，其次是一个3微通道板堆栈，和一个交叉延迟线阳极。我们描述了H33D探测器的工作台性能，以及荧光珠，量子点和活细胞获得的初步成像结果，并讨论了未来一代探测器的应用，用于单分子成像和生物分子相互作用的高通量研究。(c)2006 Elsevier B.V.保留所有权利。
We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross-delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results obtained with fluorescent beads, quantum dots and live cells and discuss applications of future generation detectors for single-molecule imaging and high-throughput study of biomolecular interactions. (c) 2006 Elsevier B.V. All rights reserved.