Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment.
Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment.
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稀硫酸预浸与即时弹射汽爆预处理相结合提高烟秆烟碱脱除率和乙醇发酵能力
DOI：
10.3389/fbioe.2021.763549
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发表时间：
2021
影响因子：
5.7
通讯作者：
Song A
中科院分区：
文献类型：
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作者：
Zhang H;Fu C;Ren T;Xie H;Mao G;Wang Z;Wang F;Song A
烟秆中的烟碱对纤维素酶活性和微生物发酵能力表现出明显的抑制作用，严重阻碍了烟秆的利用。采用稀硫酸预浸泡烟秆来提高烟秆预处理的瞬间弹射蒸汽爆炸（ICSE）性能。预浸有利于打破烟梗的顽固结构，降低烟碱含量，解除对纤维素酶活性和微生物代谢的抑制，促进酶解和乙醇发酵的性能。优化的 0.8% 硫酸 (w/w) 预浸泡集成 ICSE 预处理可实现烟梗中 85.54% 的尼古丁去除率；同时，与单独的ICSE预处理相比，预处理后的烟梗酶解总糖浓度从33.40 g/L增加到53.81 g/L（干烟梗与水的比例为1:8，w/w），烧瓶中乙醇浓度从5.95 g/L增加到12.10 g/L 103.36%。最终，通过增加酶解过程中烟秆的固含量（干烟秆与水的比例为1:4，w/w），5L发酵罐中乙醇浓度达到最高23.53 g/L，烟秆水解液葡萄糖的乙醇收率达到71.40%。这些成果达到了废弃烟秆高效利用的预期目的。
The nicotine from tobacco stalk showed obvious inhibitory effect on the activity of cellulase and fermentability of microorganisms, which seriously hinders the utilization of tobacco stalk. Dilute sulfuric acid presoak of tobacco stalk was used to enhance the performance of instant catapult steam explosion (ICSE) for tobacco stalk pretreatment. The presoak was beneficial to break the recalcitrant structure of tobacco stalk, reduce nicotine content to relieve the inhibition on the activity of cellulase and metabolism of microorganisms, and promote the performance of enzymatic hydrolysis and ethanol fermentation. The optimized 0.8% sulfuric acid (w/w) presoak–integrated ICSE pretreatment resulted in 85.54% nicotine removal from tobacco stalk; meanwhile, the total sugar concentration from enzymatic hydrolysis of pretreated tobacco stalk increased from 33.40 to 53.81 g/L (the ratio of dry tobacco stalk to water was 1:8, w/w), ethanol concentration increased 103.36% from 5.95 to 12.10 g/L in flask, compared with separate ICSE pretreatment. Finally, the ethanol concentration achieved the highest 23.53 g/L in a 5-L fermenter with the ethanol yield from the glucose of tobacco stalk hydrolysate achieving 71.40% by increasing the solid loading of the tobacco stalk in the enzymatic hydrolysis process (the ratio of dry tobacco stalk to water was 1:4, w/w). These results achieved the expected purpose of efficient utilization of discarded tobacco stalk.
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