bFGF-chitosan scaffolds effectively repair 20 mm sciatic nerve defects in adult rats
bFGF-chitosan scaffolds effectively repair 20 mm sciatic nerve defects in adult rats
复制标题
bFGF-壳聚糖支架有效修复成年大鼠20毫米坐骨神经缺损
DOI：
10.1088/1748-605x/abd9dc
复制
发表时间：
2021-03-01
影响因子：
4
通讯作者：
Li, Xiaoguang
中科院分区：
文献类型：
--
作者：
Liu, Fadong;Hao, Fei;Li, Xiaoguang
周围神经损伤的修复仍是临床面临的一大挑战。自体神经移植是治疗长距离周围神经缺损的金标准，但这种方法仍然存在供体部位高发病率和缺乏匹配供体的问题。本研究采用负载控释碱性成纤维细胞生长因子（bFGF）的新型壳聚糖支架（CS）修复成年大鼠坐骨神经20 mm缺损。扫描电镜观察bFGF-CS的超微结构。采用拉伸试验机和纳米压痕法对其力学性能进行了评价。采用霍乱毒素b亚单位（CTB）示踪、坐骨神经功能指数、肌电图、再生神经和运动终板免疫荧光染色评价大鼠坐骨神经再生。结果表明，bFGF-CS的结构和力学性能有利于坐骨神经的再生。术后12周，bFGF-CS促进大鼠坐骨神经再生。CTB成功穿过坐骨神经缺损区到达坐骨神经细胞体。重建运动终板，促进行为恢复。结果表明，bFGF-CS是修复20 mm坐骨神经缺损的有效手段，具有很大的临床应用潜力。
The repair of peripheral nerve injury is still a great challenge in clinic. Autologous nerve transplantation is the gold standard for the treatment of long-distance peripheral nerve defects, but this method remains associated with high morbidity of the donor site and lack of matching donor. In this study, a novel chitosan scaffold (CS) loaded with control-released basic fibroblast growth factor (bFGF) was used to repair 20 mm sciatic nerve defects in adult rat. The ultrastructure of bFGF-CS was observed by scanning electron microscope. The tensile tester and nano-indentation were used to evaluate its mechanical properties. Cholera toxin B-subunit (CTB) tracing, sciatic nerve function index, electromyography, immunofluorescence staining of regenerated nerve and motor endplate were used to evaluate the regeneration of sciatic nerve in rats. The results showed that the structure and mechanical properties of bFGF-CS was beneficial to the regeneration of sciatic nerve. At 12 weeks after operation, bFGF-CS facilitated sciatic nerve regeneration in rat. CTB successfully crossed the sciatic nerve defect area to reach the cell body of sciatic nerve. The motor endplate was reconstructed, thus promoting the behavioral recovery. These findings suggest that the bFGF-CS provides an effective means of repairing 20 mm sciatic nerve defects and shows great potential for clinical application.