Characterizing Semaphorin Signaling in vivo using C. elegans
Characterizing Semaphorin Signaling in vivo using C. elegans
复制标题
使用秀丽隐杆线虫表征体内信号蛋白信号传导
DOI：
10.1007/978-1-4939-6448-2_34
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发表时间：
2017
影响因子：
--
通讯作者：
Takagi S
中科院分区：
文献类型：
--
作者：
Nukazuka A;Takagi S
一种小型模式动物秀丽隐杆线虫特别适合于遗传分析，但在这种生物体中，细胞类型特异性生物化学是一项艰巨的任务。在这里，我们描述的技术利用transgenicC。表达表位标记的蛋白质的线虫菌株，用于分析在特定发育阶段的少量特定细胞群中的生物化学事件，例如蛋白质磷酸化和蛋白质复合物的形成。这些技术对阐明C. eleganssemaphorin-plexin信号系统通过调节TOR信号及其下游靶点来调节表皮形态发生。
A small model animalCaenorhabditis elegansis particularly suitable for genetic analysis, but cell-type-specific biochemistry is a formidable task in this organism. Here we describe techniques utilizing transgenicC. elegansstrains expressing epitope-tagged proteins for analyzing biochemical events, such as protein phosphorylation and formation of protein complex, in a small number of a specific group of cells at a defined stage of development. The techniques are useful for elucidating thatC. eleganssemaphorin-plexin signaling systems regulate epidermal morphogenesis through modulating TOR signaling and its downstream targets.