Raman imaging to investigate ultrastructure and composition of plant cell walls:: distribution of lignin and cellulose in black spruce wood (Picea mariana)
Raman imaging to investigate ultrastructure and composition of plant cell walls:: distribution of lignin and cellulose in black spruce wood (Picea mariana)
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DOI：
10.1007/s00425-006-0295-z
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发表时间：
2006-10-01
期刊：
影响因子：
4.3
通讯作者：
Agarwal, Umesh P.
中科院分区：
文献类型：
--
作者：
Agarwal, Umesh P.
从植物生物学和化学以及商业利用的角度来看，详细了解维管植物细胞壁的结构组织是重要的。采用最先进的633 nm激光共聚焦拉曼显微镜原位测定了黑云杉木材横截面细胞壁组分的分布。从形态上不同的细胞壁区域的化学信息，并产生木质素和纤维素的空间分布的拉曼图像。细胞角（CC）的木质素含量最高，而复合中层（CmL）与次生壁（S2和S2-S3）的木质素含量差异不显著。使用1，650 cm（-1）p带生成的图像显示，松柏醛和松柏醇的分布遵循木质素的分布，因此没有特定的细胞壁层/区域富含烯属残留物。相反，纤维素分布表现出相反的模式-低浓度在CC和CmL和高的S2区。然而，纤维素浓度变化显着，在某些地区，和木质素和纤维素的浓度都很高，在其他地区。虽然木质素和纤维素分布的强度图目前仅解释在浓度差异方面，取向的影响需要仔细考虑，以揭示木材细胞壁的组织。
A detailed understanding of the structural organization of the cell wall of vascular plants is important from both the perspectives of plant biology and chemistry and of commercial utilization. A state-of-the-art 633-nm laser-based confocal Raman microscope was used to determine the distribution of cell wall components in the cross section of black spruce wood in situ. Chemical information from morphologically distinct cell wall regions was obtained and Raman images of lignin and cellulose spatial distribution were generated. While cell corner (CC) lignin concentration was the highest on average, lignin concentration in compound middle lamella (CmL) was not significantly different from that in secondary wall (S2 and S2-S3). Images generated using the 1,650 cm(-1)p band showed that coniferaldehyde and coniferyl alcohol distribution followed that of lignin and no particular cell wall layer/region was therefore enriched in the ethylenic residue. In contrast, cellulose distribution showed the opposite pattern-low concentration in CC and CmL and high in S2 regions. Nevertheless, cellulose concentration varied significantly in some areas, and concentrations of both lignin and cellulose were high in other areas. Though intensity maps of lignin and cellulose distributions are currently interpreted solely in terms of concentration differences, the effect of orientation needs to be carefully considered to reveal the organization of the wood cell wall.