Molecular cloning, expression analysis of insulin-like growth factor I (IGF-I) gene and IGF-I serum concentration in female and male Tongue sole (Cynoglossus semilaevis)
Molecular cloning, expression analysis of insulin-like growth factor I (IGF-I) gene and IGF-I serum concentration in female and male Tongue sole (Cynoglossus semilaevis)
复制标题
雌雄半滑舌鳎胰岛素样生长因子I（IGF-I）基因的分子克隆、表达分析及IGF-I血清浓度
DOI：
10.1016/j.cbpb.2011.08.008
复制
发表时间：
2011-12-01
影响因子：
2.2
通讯作者：
Tang, Qi-Sheng
中科院分区：
文献类型：
--
作者：
Ma, Qian;Liu, Shu-Fang;Tang, Qi-Sheng
胰岛素样生长因子 I (IGF-I) 是一种多肽激素，可调节脊椎动物各个发育阶段的生长。为了探讨半滑舌鳎(Cynoglossus semilaevis)有性生长二态性的机制，进行了IGF-I基因的分子克隆、表达分析和IGF-I血清浓度分析。结果，IGF-I cDNA序列为911bp，包含564bp的开放阅读框(ORF)，编码187个氨基酸的蛋白质。使用来自雌性、正常雄性和超大雄性成虫的 14 个组织分析了性别特异性组织表达。 IGF-I mRNA主要在肝脏中表达，雌性和超大雄性的IGF-I表达水平分别是正常雄性的1.9和10.2倍。还利用半滑鼠全同胞家族检测了生命早期IGF-I mRNA表达的性别差异，并且在10至410日龄的所有27个采样点均检测到了IGF-I mRNA。 190 日龄鱼后检测到 IGF-I mRNA 增加。 190日龄后，雌性IGF-I mRNA水平显着高于雄性（P
Insulin-like growth factor I (IGF-I) is a polypeptide hormone that regulates growth during all stages of development in vertebrates. To examine the mechanisms of the sexual growth dimorphism in the Tongue sole (Cynoglossus semilaevis), molecular cloning, expression analysis of IGF-I gene and IGF-I serum concentration analysis were performed. As a result, the IGF-I cDNA sequence is 911 bp, which contains an open reading frame (ORF) of 564 bp encoding a protein of 187 amino acids. The sex-specific tissue expression was analyzed by using 14 tissues from females, normal males and extra-large male adults. The IGF-I mRNA was predominantly expressed in liver, and the IGF-I expression levels in females and extra-large males were 1.9 and 10.2 times as much as those in normal males, respectively. Sex differences in IGF-I mRNA expressions at early life stages were also examined by using a full-sib family of C. semilaevis, and the IGF-I mRNA was detected at all of the 27 sampling points from 10 to 410 days old. An increase in IGF-I mRNA was detected after 190 day old fish. The significantly higher levels of IGF-I mRNA in females were observed after 190 days old in comparison with males (P