Quantitative and qualitative evaluation of toxic metals and trace elements in the tissues of renal cell carcinoma compared with the adjacent non-cancerous and control kidney tissues
Quantitative and qualitative evaluation of toxic metals and trace elements in the tissues of renal cell carcinoma compared with the adjacent non-cancerous and control kidney tissues
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DOI：
10.1007/s11356-020-09402-y
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发表时间：
2020-08-01
影响因子：
5.8
通讯作者：
Ali-El-Dein, Bedeir
中科院分区：
文献类型：
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作者：
Abdel-Gawad, Mahmoud;Elsobky, Emad;Ali-El-Dein, Bedeir
有毒金属和微量元素（TMTE）与几种人类癌症的发展有关。许多报告已经记录了一些TMTE和肾细胞癌之间的关联。在这项工作中，我们评估的存在（定性）和评价的浓度（定量）的22 TMTE在三组肾组织样本：肾细胞癌（RCC），相邻的非癌，并控制肾脏组织的尸体。肾癌根治术后即刻取肾癌组织及癌旁组织标本75例，保存于10%福尔马林溶液中。12例正常肾组织标本，年龄和性别匹配的尸体，谁死于非癌症的原因，收集和作为对照。通过使用电感耦合等离子体发射光谱法（ICP-OES）技术对所有组织样本进行TMTE浓度（每个样本中有22种元素）评价。肿瘤、病理组织学、分期、分级与TTE浓度及类型相关。结果显示：肾细胞癌组织学类型：透明细胞型35例（21.5%），嫌色细胞型22例（13.5%），乳头状型7例（4.5%），嗜酸细胞瘤型5例（3.1%），未分类型6例（3.7%）。ICP-OES显示，与相邻的非癌组织和对照组织相比，肿瘤（RCC）组织具有更高浓度的9种TMTE（Ca、Cd、K、Mg、Mn、Na、Pb、S和Sr）。癌旁肾组织中Fe、K、Na含量最高。对照组肾组织中Cu、Zn、Mo、B含量高于癌组织和癌旁组织。女性患者的肾脏非癌组织中锌和铜的浓度较高。年龄越小，癌旁组织中B浓度越高，癌组织中Cu浓度越高。镉浓度最高的嫌色细胞型RCC与其他亚型相比。TTE浓度与肾细胞癌的病理分期无关。
Toxic metals and trace elements (TMTE) are linked to the development of several human cancers. Many reports have documented the association between some TMTE and renal cell carcinoma. In this work, we assessed the presence (qualitative) and evaluated the concentration (quantitative) of 22 TMTE in three groups of kidney tissue samples: renal cell carcinoma (RCC), adjacent non-cancerous, and control kidney tissues from cadavers. A total of 75 paired specimens of RCC and adjacent non-cancerous tissues were harvested immediately after radical nephrectomy and preserved in 10% diluted formalin solution. Twelve specimens, age- and sex-matched from the normal kidney tissue of the cadavers, who died from non-cancerous reasons, were collected and served as control. All tissue specimens were subjected to evaluation of TMTE concentration (22 elements in each specimen) by using the inductively coupled plasma optical emission spectrometry (ICP-OES) technique. The tumor, histopathology, stage, and grade were correlated with the concentration and types of TMTE. The results showed that the histological types of RCC were as follows: clear cell type in 35 (21.5%), chromophobe 22 (13.5%), papillary 7 (4.5%), oncocytoma 5 (3.1%), and unclassified 6 (3.7%). ICP-OES revealed that tumorous (RCC) tissues had a higher concentration of 9 TMTE (Ca, Cd, K, Mg, Mn, Na, Pb, S, and Sr) compared with both the adjacent non-cancerous and control tissue. The adjacent non-cancerous kidney tissues showed the highest concentration of Fe, K, and Na. The control of kidney tissues from cadavers had the highest level of Cu, Zn, Mo, and B compared with the cancerous and adjacent non-cancerous tissues. Female patients had higher concentrations of Zn and Cu in the non-cancerous tissues of their kidneys. Younger patients had a higher concentration of B in the adjacent non-cancerous, and higher Cu in the cancerous tissues. Cadmium concentration was highest in the chromophobe cell type of RCC compared with other subtypes. There was no correlation between the TMTE concentration and the pathological stage of RCC.