Cytoplasmic polyadenylation element-like sequences are involved in dendritic targeting of BDNF mRNA in hippocampal neurons
Cytoplasmic polyadenylation element-like sequences are involved in dendritic targeting of BDNF mRNA in hippocampal neurons
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DOI：
10.1016/j.febslet.2010.06.040
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发表时间：
2010-08-04
期刊：
影响因子：
3.5
通讯作者：
Yoneda, Yoshihiro
中科院分区：
文献类型：
--
作者：
Oe, Souichi;Yoneda, Yoshihiro
已知有几个mRNAs靶向于海马神经元中的树突。在本研究中，我们发现脑源性神经营养因子(BDNF)mRNA在短3‘非翻译区(UTR)有两个不同的作用树突状靶向元件：一个是构成元件，一个是活性依赖元件。此外，短3‘非编码区中一系列细胞质多聚腺苷酸化元件(CPE)样序列的缺失抑制了组成性和活性依赖的树突状细胞靶向。除与胞浆多聚腺苷酸化元件结合蛋白-1(CPEB-1)相互作用外，去极化还能促进CPEB-1向活性依赖靶向元件的募集。这些结果表明，CPE样序列参与了BDNF mRNA的活性依赖和结构性树突状靶向。(C)2010年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Several mRNAs are known to be targeted to dendrites in hippocampal neurons. In this study, we show that brain-derived neurotrophic factor (BDNF) mRNA has two distinct as-acting dendritic targeting elements in the short 3' untranslated region (UTR): a constitutive element and an activity-dependent one. Moreover, deletion of serial cytoplasmic polyadenylation element (CPE)-like sequences in the short 3'UTR suppressed both constitutive and activity-dependent dendritic targeting. In addition to the interaction with cytoplasmic polyadenylation element binding protein-1 (CPEB-1), depolarization enhanced CPEB-1 recruitment to the activity-dependent targeting element. These results suggest that CPE-like sequences are involved in the activity-dependent as well as constitutive dendritic targeting of BDNF mRNA. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.