The Prosencephalon Has the Capacity to Differentiate into the Optic Tectum: Analysis by Chick‐Specific Monoclonal Antibodies in Quail‐Chick‐Chimeric Brains
The Prosencephalon Has the Capacity to Differentiate into the Optic Tectum: Analysis by Chick‐Specific Monoclonal Antibodies in Quail‐Chick‐Chimeric Brains
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前脑具有分化为视顶盖的能力：鹌鹑-小鸡-嵌合脑中小鸡特异性单克隆抗体的分析
DOI：
10.1111/j.1440-169x.1988.00717.x
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发表时间：
1988
期刊：
影响因子：
4.6
通讯作者：
H. Fujisawa
中科院分区：
文献类型：
--
作者：
Harukazu Nakamura;S. Takagi;T. Tsuji;K. Matsui;H. Fujisawa
将鹌鹑胚前脑鼻翼板异位移植到鸡胚中脑鼻翼板7-10体期。鸡和鹌鹑嵌合脑细胞经Feulgen - Rossenbeck染色和/或免疫组织化学染色，用物种特异性单克隆抗体MAb‐37F5识别鸡脑细胞的细胞质成分。通过单克隆抗体MAb39‐B11和MAb‐29B8研究了移植体和宿主之间的神经连接，MAb39‐B11能识别鸡神经纤维上的物种特异性抗原，MAb‐29B8能对鸡和鹌鹑的160 kD神经纤维起反应。
The alar plate of the prosencephalon of the quail embryo was heterotopically transplanted into the alar plate of the mesencephalon of the chick embryo at the 7–10 somite stage. Chick and quail cells in chimeric brains were distinguished after Feulgen‐Rossenbeck staining and/or immunohistochemical staining with a species specific monoclonal antibody MAb‐37F5 which recognized cytoplasmic components of chick brain cells. Neural connections between the transplant and the host were studied by monoclonal antibodies, MAb39‐B11, which recognizes a species specific antigen on chick nerve fibers, and MAb‐29B8, which reacts to 160 kD neurofilaments of both chick and quail.