Association between genetic variants of microRNA-21 and microRNA-155 and systemic lupus erythematosus: A case-control study from a Chinese population.
Association between genetic variants of microRNA-21 and microRNA-155 and systemic lupus erythematosus: A case-control study from a Chinese population.
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microRNA-21和microRNA-155的遗传变异与系统性红斑狼疮之间的关联：来自中国人群的病例对照研究
DOI：
10.1002/jcla.24518
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发表时间：
2022-07
影响因子：
2.7
通讯作者：
Huang, Huayi
中科院分区：
文献类型：
--
作者：
Wang, Rong;Wei, Anji;Zhang, Yingjie;Xu, Guidan;Nong, Xuejuan;Liu, Chunhong;Zeng, Yonglong;Huang, Huatuo;Pang, Xiaoxia;Wei, Wujun;Wang, Chunfang;Huang, Huayi
系统性红斑狼疮（SLE）是一种常见的自身免疫性疾病，其发病机制尚不清楚。遗传物质的改变被认为在SLE的发展中起作用。该研究评估了microRNA-21（miR-21）和microRNA-155（miR-155）的遗传变异与SLE之间的关联。SNaPshot基因分型方法用于检测患者和对照组中选定的SNPs基因型。使用逆转录-定量聚合酶链反应（RT-qPCR）分析miR-21和miR-155的表达。应用HaploReg V4.1和Regulome DB V2.0软件对SNPs进行功能注释和生物学效应分析。采用Hardy-Weinberg平衡检验进行统计学分析，并通过logistic回归评估比值比（OR）和95%置信区间（CI）。rs767649位点TA基因型的分布差异有统计学意义（TA vs. T/T：OR = 0.68，95%CI，0.48-0.95，p = 0.026）。T/A + A/A存在显著差异（T/A + A/A vs. T/T：OR = 0.68，95%CI，0.49-0.94，p = 0.020）。T等位基因在SLE患者补体抑制中的分布差异有统计学意义（T vs. A：OR = 0.67，95%CI，0.47-0.95，p = 0.026）。阳性和阴性SSB抗体（抗SSB）之间rs 13137的遗传变异存在显著差异（T vs. A：OR = 0.67，95%CI，0.47-0.95，p = 0.026; T/A + T/T vs. AA：OR = 2.23，1.18-4.49，p = 0.013）。患者中miR-21和miR-155的表达水平显著高于对照组（p < 0.001）。这项研究提供了新的见解，即rs767649和rs 13137的遗传变异与SLE的易感性相关。rs767649和rs 13137基因变异与系统性红斑狼疮的相关性研究。
Systemic lupus erythematosus (SLE) is a common autoimmune disease, and its pathogenesis remains unclear. The alteration of genetic materials is believed to play a role in SLE development. This study evaluated the association between the genetic variants of microRNA‐21 (miR‐21) and microRNA‐155 (miR‐155) and SLE. The SNaPshot genotyping method was used to detect the genotypes of selected SNPs in patients and controls. The expression of miR‐21 and miR‐155 was analyzed using reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). The functional annotation and the biological effects of SNPs were assessed by HaploReg V4.1 and Regulome DB V2.0 software. The Hardy–Weinberg equilibrium test was used to gather statistics, and odds ratios (ORs) and 95% confidence intervals (CIs) were evaluated by logistic regression. The distribution difference of TA genotype in rs767649 was observed (TA vs. T/T: OR = 0.68, 95%CI, 0.48–0.95, p = 0.026). There was a significant difference in the T/A + A/A (T/A + A/A vs. T/T: OR = 0.68, 95%CI, 0.49–0.94, p = 0.020). A significant difference in T allele distribution was found in the depressed complement of SLE (T vs. A: OR = 0.67, 95%CI, 0.47–0.95, p = 0.026). There were significant differences in genetic variants of rs13137 between the positive and the negative SSB antibodies (Anti‐SSB) (T vs. A: OR = 0.67, 95%CI, 0.47–0.95, p = 0.026; T/A + T/T vs. AA: OR = 2.23, 1.18–4.49, p = 0.013). The expression levels of miR‐21 and miR‐155 were significantly higher in patients than in controls (p < 0.001). This study provides novel insight that genetic variants of rs767649 and rs13137 are associated with susceptibility to SLE. Association between genetic variants of rs767649 and rs13137 with systemic lupus erythematosus.
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DOI：
10.4049/jimmunol.0803560
发表时间：
2009-04-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lu TX;Munitz A;Rothenberg ME
通讯作者：
Rothenberg ME
DOI：
10.4049/jimmunol.1101235
发表时间：
2011-09-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lu TX;Hartner J;Lim EJ;Fabry V;Mingler MK;Cole ET;Orkin SH;Aronow BJ;Rothenberg ME
通讯作者：
Rothenberg ME
DOI：
10.1002/art.39403
发表时间：
2016-01
期刊：
Arthritis & rheumatology (Hoboken, N.J.)
影响因子：
--
作者：
Demirci FY;Wang X;Kelly JA;Morris DL;Barmada MM;Feingold E;Kao AH;Sivils KL;Bernatsky S;Pineau C;Clarke AE;Ramsey-Goldman R;Vyse TJ;Gaffney PM;Manzi S;Kamboh MI
通讯作者：
Kamboh MI
影响因子：
2.5
作者：
Khoshmirsafa, Majid;Kianmehr, Nahid;Shekarabi, Mehdi
通讯作者：
Shekarabi, Mehdi
系统性红斑狼疮
影响因子：
81.5
作者：
Kaul, Arvind;Gordon, Caroline;Hughes, Graham
通讯作者：
Hughes, Graham