Methyloversatilis thermotolerans sp nov., a novel thermotolerant facultative methylotroph isolated from a hot spring
Methyloversatilis thermotolerans sp nov., a novel thermotolerant facultative methylotroph isolated from a hot spring
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DOI：
10.1099/ijs.0.055046-0
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发表时间：
2014-01-01
影响因子：
2.8
通讯作者：
Trotsenko, Yuri A.
中科院分区：
文献类型：
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作者：
Doronina, Nina V.;Kaparullina, Elena N.;Trotsenko, Yuri A.
对一株新分离的兼性甲基营养细菌(3T(T))进行了研究。该分离物的细胞为革兰氏染色阴性、无孢子生、不活动的杆状，通过二次分裂增殖。该菌株利用甲醇、甲胺和多种多碳化合物作为碳源和能源。生长环境为pH 6.5-8.5(最适为7.0-7.5)和10-45℃(最适为30-37℃)。甲醇培养细胞的主要脂肪酸为C-16：1、omega 7c和C-16：0。磷脂主要为磷脂酰乙醇胺和磷脂酰甘油。辅基苯二酚主要为Q-8。菌株3T(T)具有吡咯喹啉醌(PQQ)连接的甲醇脱氢酶，并通过丝氨酸循环在甲醛和二氧化碳水平上同化C-1单位。该菌株DNA G+C含量为63.6mol%(T-m)。根据16S rRNA基因序列的相似性(98.1%)和较低的DNADNA亲缘关系(30%)，以及生理生化特征，该分离物被归类为该属的一个新种，命名为耐热耐热菌3t(T)(=VKM B-2692(T)=CCUG 61694(T)=DSM 25156(T))。
A newly isolated facultatively methylotrophic bacterium (strain 3t(T)) was investigated. Cells of the isolate were Gram-stain-negative, asporogenous, non-motile rods that multiplied by binary fission. The strain utilized methanol, methylamine and a variety of multicarbon compounds as carbon and energy sources. Growth occurred at pH 6.5-8.5 (optimally at 7.0-7.5) and at 10-45 degrees C (optimally at 30-37 degrees C). The major fatty acids of methanol-grown cells were C-16:1 omega 7c and C-16:0. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol. The major ubiquinone was Q-8. Strain 3t(T) possessed pyrroloquinoline quinone (PQQ)-linked methanol dehydrogenase and assimilated C-1 units at the level of formaldehyde and CO2 via the serine cycle. The DNA G+C content of the strain was 63.6 mol% (T-m). On the basis of 16S rRNA gene sequence similarity (98.1%) and rather low DNA-DNA relatedness (30%) with the type strain of the type species of the genus Methyloversatilis (Methyloversatilis universalis FAM5(T)), and physiological and biochemical characteristics, the isolate was classified as a representative of a new species of the genus and named Methyloversatilis thermotolerans 3t(T) (=VKM B-2692(T)=CCUG 61694(T)=DSM 25156(T)).