delta Opioid extends hypothermic preservation time of the lung.
delta Opioid extends hypothermic preservation time of the lung.
复制标题
δ阿片类药物可延长肺的低温保存时间。
DOI：
10.1016/s0022-5223(96)70424-5
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Chien,S
中科院分区：
文献类型：
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作者：
Wu,G;Zhang,F;Salley,RK;Diana,JN;Su,TP;Chien,S
为了验证δ阿片样物质DADLE（[D-Ala 2，D-Leu 5]-脑啡肽）在低温肺保存过程中可以保护组织免受缺血性损伤的假设，我们研究了三组大鼠。在组1（n = 8）中，在收获后立即研究肺功能。在第2组（n = 8）中，用4º C Euro-Collins溶液冲洗肺并保存24小时。在第3组（n = 8）中，用4º C Euro-Collins溶液加DADLE（1 mg/kg）冲洗肺并保存24小时。肺功能的研究采用活体大鼠灌流模型。来自宿主大鼠的静脉血灌注离体肺的肺动脉。用滚子泵将来自分离的肺的血液返回到宿主大鼠的颈动脉。第2组在灌注30分钟内发生严重的肺水肿、出血和闭塞性肺动脉阻力。在第1组和第3组中进行了超过60分钟的灌注研究。组2的肺血流量低于组1或组3。肺血管阻力2组明显高于1、3组（P < 0.05）。2组气道压力和气道阻力明显高于1组和3组（P < 0.05）。灌流20分钟后，第3组的气道阻力也高于第1组（P < 0.05）。离体肺静脉氧分压2组低于1组和3组（P < 0.05）。第2组的肺泡-动脉血氧分压差明显高于第1组和第3组（P < 0.05）。灌注后肺组织湿/干重比在组2中比在组1和3中高得多。实验结果首次清楚地表明，DADLE能有效地提高大鼠低温肺保存的效果。（J THORAC CARDIOVASC SURG 1996;111：259-67）
To test the hypothesis that a delta opioid, DADLE ([D-Ala2, D-Leu5]-enkephalin), could protect tissue from ischemic damage during hypothermic lung preservation, we studied three groups of rats. In group 1 ( n = 8), lung function was studied immediately after harvesting. In group 2 ( n = 8), the lung was flushed with 4º C Euro-Collins solution and preserved for 24 hours. In group 3 ( n = 8), the lung was flushed with 4º C Euro-Collins solution plus DADLE (1 mg/kg) and preserved for 24 hours. Lung function was studied by using a living rat perfusion model. Venous blood from the host rat perfused the pulmonary artery of the isolated lung. Blood from the isolated lung was returned to the carotid artery of the host rat with a roller pump. Severe pulmonary edema, hemorrhage, and occlusive pulmonary artery resistance occurred in group 2 within 30 minutes of perfusion. Perfusion studies were carried out for more than 60 minutes in groups 1 and 3. Pulmonary blood flow was lower in group 2 than in either group 1 or group 3. Pulmonary vascular resistance was much higher in group 2 than in groups 1 and 3 ( p < 0.05). Airway pressure and airway resistance were much higher in group 2 than in groups 1 and 3 ( p < 0.05). Airway resistance was also higher in group 3 than in group 1 after 20 minutes of perfusion ( p < 0.05). Oxygen tensions from the pulmonary vein of the isolated lung in group 2 were lower than those in groups 1 and 3 ( p < 0.05). Alveolar-arterial oxygen difference was much higher in group 2 than in groups 1 and 3 ( p < 0.05). Lung tissue wet/dry weight ratio after perfusion was much higher in group 2 than in groups 1 and 3. The results clearly show, for the first time, that DADLE can effectively enhance hypothermic lung preservation in rats.(J THORAC CARDIOVASC SURG 1996;111:259-67)